AUTHOR=Liu Lu , Xiao Shunli , Wang Yilin , Wang Yufang , Liu Lei , Sun Zhengxiao , Zhang Qian , Yin Xiaojie , Liao Fulong , You Yun , Zhang Xuguang
TITLE=Water-soluble tomato concentrate modulates shear-induced platelet aggregation and blood flow in vitro and in vivo
JOURNAL=Frontiers in Nutrition
VOLUME=9
YEAR=2022
URL=https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2022.961301
DOI=10.3389/fnut.2022.961301
ISSN=2296-861X
ABSTRACT=
Water-soluble tomato concentrate (WSTC), extracted from mature tomatoes, is the first health product in Europe that has been approved “to help maintain normal platelet activity to maintain healthy blood flow.” We hypothesized that WSTC might exert an influence on blood flow shear stress-induced platelet aggregation (SIPA) and in turn maintains healthy blood flow. We used a microfluidic system to measure the effects of WSTC on SIPA in vitro. We also used the strenuous exercise rat model and the κ-carrageenan-induced rat tail thrombosis model to demonstrate the effects of WSTC on blood flow. WSTC significantly inhibited platelet aggregation at pathological high shear rate of 4,000 s–1 and 8,000 s–1in vitro (P < 0.05 or P < 0.01). WSTC reduced the platelet adhesion rate and increased the rolling speed of platelets by inhibiting binding to Von Willebrand Factor (vWF) (P < 0.05 or P < 0.01). The oral administration of WSTC for 4 weeks in strenuous exercise rats alleviated hyper-reactivity of the platelets and led to a significant reduction in the plasma levels of catecholamine and IL-6. WSTC treatment also led to a reduction in black tail length, reduced blood flow pulse index (PI) and vascular resistance index (RI), and ameliorated local microcirculation perfusion in a rat model of thrombosis. WSTC exerted obvious inhibitory effects on the platelet aggregation induced by shear flow and alleviated the blood flow and microcirculation abnormities induced by an inflammatory reaction.