AUTHOR=He Peng , Zhang Mi , Zhao Meng , Zhang Mengyao , Ma Benxu , Lv Hongyu , Han Yantao , Wu Dingtao , Zhong Zhangfeng , Zhao Wenwen 

TITLE=A Novel Polysaccharide From Chuanminshen violaceum and Its Protective Effect Against Myocardial Injury

JOURNAL=Frontiers in Nutrition

VOLUME=9

YEAR=2022

URL=https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2022.961182

DOI=10.3389/fnut.2022.961182

ISSN=2296-861X

ABSTRACT=<p>We isolated and purified a novel polysaccharide from the root of <italic>Chuanminshen violaceum</italic>, namely, <italic>Chuanminshen violaceumis</italic> polysaccharide (CVP) and confirmed its structure and molecular weight. Furthermore, <italic>in vivo</italic> experiment, CVP’s protective effect against myocardial ischemia-reperfusion (I/R) injury in mice was evidenced by significantly reducing I/R-induced myocardial infarction (MI) size, decreasing the secretion of heart damage biomarkers, and improving cardiac function. Then, the myocardial anoxia/reoxygenation (A/R) injury model was established to mimic reperfusion injury. Noticeably, ferroptosis was the major death manner for A/R-damaged H9c2 cells. Meanwhile, CVP significantly inhibited ferroptosis by decreasing intracellular Fe<sup>2+</sup> level, enhancing GPX4 expression, and suppressing lipid peroxidation to confront A/R injury. In conclusion, CVP, with a clear structure, ameliorated I/R injury by inhibiting ferroptosis.</p>