AUTHOR=Ma Li , Guo Xueliang , Yang Jiaoyang , Zeng Xiangru , Ma Kaili , Wang Lu , Sun Qi , Wang Zichao 

TITLE=Characterization and Antibacterial Activity of a Polysaccharide Produced From Sugarcane Molasses by Chaetomium globosum CGMCC 6882

JOURNAL=Frontiers in Nutrition

VOLUME=9

YEAR=2022

URL=https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2022.935632

DOI=10.3389/fnut.2022.935632

ISSN=2296-861X

ABSTRACT=<p>As a by-product of the sugar industry containing many sugars, proteins, nitrogenous materials, and heavy metals, molasses is rarely used for polysaccharide production. In the present work, a <italic>Chaetomium globosum</italic> CGMCC 6882 polysaccharide was produced from sugarcane molasses (CGP-SM) was successfully produced from sugarcane molasses. The yield of CGP-SM was 5.83 ± 0.09 g/l and its protein content was 2.41 ± 0.12% (w/w). Structural analysis showed that CGP-SM was a crystalline and amorphous polysaccharide containing rhamnose, glucosamine, galactose, glucose, mannose, fructose, and glucuronic acid in the molar ratio of 10.31: 1.14: 2.07: 59.55: 42.65: 1.92: 9.63. Meanwhile, weight-average molecular weight (Mw), number-average molecular weight (Mn), and polydispersity (Mw/Mn) of CGP-SM were 28.37 KDa, 23.66 KDa, and 1.199, respectively. Furthermore, the bacteriostatic assay indicated that CGP-SM inhibited the growth of <italic>Escherichia coli</italic> and <italic>Staphylococcus aureus</italic> in a concentration-dependent manner, and its inhibitory effect on <italic>S. aureus</italic> was higher than that of <italic>E. coli</italic>. Above all, this work provides a green method for the production of bioactive polysaccharide from sugarcane molasses.</p>