AUTHOR=Gong Ruixue , Cao Weiguo , Huang Haijun , Yu Bao , Chen Huan , Tao Wei , Luorong Quji , Luo Juan , Zhang Dan 

TITLE=Antitumor Potential and Structure Characterization of Polysaccharides From Lagotis brevituba Maxim in the Tibetan Plateau

JOURNAL=Frontiers in Nutrition

VOLUME=9

YEAR=2022

URL=https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2022.921892

DOI=10.3389/fnut.2022.921892

ISSN=2296-861X

ABSTRACT=<p>This study purified two polysaccharides (LBMPs) from <italic>Lagotis brevituba</italic> Maxim in several steps. The chemical structure of LBMP-2 was determined by HPGPC, FT-IR, IC, <sup>1</sup>H and <sup>13</sup>C NMR, AFM, SEM, and TEM. The results show that LBMP-2 was mainly composed of GalA, and the Mw of LBMP-2 is 23.799 kDa. In addition, the antioxidant activity, and the antitumor activity <italic>in vitro</italic> and <italic>in vivo</italic> were studied. LBMP-2 has excellent antioxidant and antitumor capacity. The inhibition of tumor cell proliferation <italic>in vitro</italic> may result in the inhibition of aerobic respiration and glycolysis. Tumor growth inhibition <italic>in vivo</italic> may inhibit the expression of AMPK in tumors and enhance spleen function. Compared with conventional chemotherapy drug cyclophosphamide, LBMP-2 is less harmful to the body and safer. Therefore, LBMP-2 provides a potential source of antitumor drugs.</p>