AUTHOR=Tang Zizhong , Lin Wenjie , Chen Yusheng , Feng Shiling , Qin Yihan , Xiao Yirong , Chen Hong , Liu Yuntao , Chen Hui , Bu Tongliang , Li Qinfeng , Cai Yi , Yao Huipeng , Ding Chunbang TITLE=Extraction, Purification, Physicochemical Properties, and Activity of a New Polysaccharide From Cordyceps cicadae JOURNAL=Frontiers in Nutrition VOLUME=9 YEAR=2022 URL=https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2022.911310 DOI=10.3389/fnut.2022.911310 ISSN=2296-861X ABSTRACT=

The polysaccharides from C. cicadae were extracted by ultrasonically-assisted enzymatic extraction (UAEE). Response surface analysis was used to determine the optimum parameters as follows: addition of enzymes, 0.71%; extraction temperature, 60°C; extraction time, 18 min; liquid-solid ratio, 46:1 (mL/g). The extraction yield of polysaccharide was 3.66 ± 0.87%. A novel polysaccharide fraction (JCH-a1) from C. cicadae was extracted and then purified by cellulose DEAE-32 and Sephadex G-100 anion exchange chromatography. The analysis results showed that the molar ratio of galactose, glucose, and mannose in JCH-a1 cells (60.7 kDa) was 0.89:1:0.39. JCH-a1 with a triple helix contains more α-glycosides and has strong thermal stability. Moreover, JCH-a1 showed strong antioxidant activity and acted as a strong inhibitor of α-glucosidase in vitro. In addition, JCH-a1 can prolong the lifespan of C. elegans. The present study might provide a basis for further study of JCH-a1 as an antioxidant and hypoglycemic food or drug.