AUTHOR=Zhao Keyan , Chen Rulong , Duan Wenhui , Meng Lanhuan , Song Hongmiao , Wang Qing , Li Jiangkuo , Xu Xiangbin 

TITLE=Chilling injury of tomato fruit was alleviated under low-temperature storage by silencing Sly-miR171e with short tandem target mimic technology

JOURNAL=Frontiers in Nutrition

VOLUME=9

YEAR=2022

URL=https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2022.906227

DOI=10.3389/fnut.2022.906227

ISSN=2296-861X

ABSTRACT=<p>In this study, the role of Sly-miR171e on post-harvest cold tolerance of tomato fruit was researched. The results showed that overexpression of Sly-miR171e (miR171e-OE) promoted postharvest chilling injury (CI) of tomato fruit at the mature red (MR) and mature green (MG) stage. Contrasted with the wild type (WT) and miR171e-OE fruit, the knockdown of Sly-miR171e (miR171e-STTM) showed a lower CI index, lower hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) content, and higher fruit firmness after harvest. In the fruit of miR171e-STTM, the expression level of <italic>GRAS24, CBF1, GA2ox1</italic>, and <italic>COR</italic>, and the GA3 content were ascended, while the expression levels of <italic>GA20ox1</italic> and <italic>GA3ox1</italic> were descended. The research demonstrated that CI in tomato fruit was alleviated at low temperature storage by silencing Sly-miR171e with short tandem target mimic (STTM) technology. Furthermore, it also provided helpful information for genetic modification of miR171e and control of CI in the postharvest fruit.</p>