AUTHOR=Xu Shengyu , Dong Yanpeng , Chen Sirun , Liu Yalei , Li Zimei , Jia Xinlin , Briens Mickael , Jiang Xuemei , Lin Yan , Che Lianqiang , Zhuo Yong , Li Jian , Feng Bin , Fang Zhengfeng , Wang Jianping , Ren Zhihua , Wu De 

TITLE=2-Hydroxy-4-Methylselenobutanoic Acid Promotes Follicle Development by Antioxidant Pathway

JOURNAL=Frontiers in Nutrition

VOLUME=9

YEAR=2022

URL=https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2022.900789

DOI=10.3389/fnut.2022.900789

ISSN=2296-861X

ABSTRACT=<p>Selenium (Se) is assumed to promote the follicle development by attenuating oxidative stress. The current study was developed to evaluate the effects of dietary 2-hydroxy-4-methylselenobutanoic acid (HMSeBA) supplementation on the follicle development <italic>in vivo</italic> and on the function of ovarian granulosa cells (GCs) <italic>in vitro</italic>. Thirty-six gilts were randomly assigned to fed control diet (CON), Na<sub>2</sub>SeO<sub>3</sub> diet (0.3 mg Se/kg) or HMSeBA diet (0.3 mg Se/kg). The results showed that HMSeBA and Na<sub>2</sub>SeO<sub>3</sub> supplementation both increased the total selenium content in liver and serum compared with control, while HMSeBA increased the total selenium content in liver compared with Na<sub>2</sub>SeO<sub>3</sub> group. HMSeBA tended to increase the total selenium content in ovary compared with control. HMSeBA and Na<sub>2</sub>SeO<sub>3</sub> supplementation both increased the weight of uteri in gilts at the third estrus. Moreover, HMSeBA supplementation down-regulated the gene expression of growth differentiation factor-9 (<italic>GDF-9</italic>) and bone morpho-genetic protein-15 (<italic>BMP-15</italic>) in cumulus-oocyte complexes (COCs). HMSeBA supplementation decreased malondialdehyde (MDA) content in serum, liver and ovary, increased activity of T-AOC in liver, TXNRD in ovary and GPX in serum, liver and ovary, while up-regulated the liver <italic>GPX2, SOD1</italic> and <italic>TXNRD1</italic>, ovarian <italic>GPX1</italic> gene expression. <italic>In vitro</italic>, HMSeBA treatment promoted GCs' proliferation and secretion of estradiol (E<sub>2</sub>). HMSeBA treatment increased the activity of T-AOC, T-SOD, GPX, TXNRD and decreased MDA content in GCs <italic>in vitro</italic>. Meanwhile, HMSeBA treatment up-regulated <italic>SOD2</italic> and <italic>GPX1</italic> gene expression in GCs <italic>in vitro</italic>. In conclusion, HMSeBA supplementation is more conducive to promoting follicle development by antioxidant pathway.</p>