AUTHOR=Wang Xiaomei , Xu Xiaogang , Mao Genxiang , Guo Yue , Wang Guangce , Sun Xue , Xu Nianjun , Zhang Zhongshan 

TITLE=Structural Characterization of Gracilariopsis lemaneiformis Polysaccharide and Its Property in Delaying Cellular Senescence

JOURNAL=Frontiers in Nutrition

VOLUME=9

YEAR=2022

URL=https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2022.876992

DOI=10.3389/fnut.2022.876992

ISSN=2296-861X

ABSTRACT=<p>The sulfated polysaccharide was isolated from the purified <italic>G. lemaneiformis</italic> polysaccharide (PGP), and its property in delaying H<sub>2</sub>O<sub>2</sub>-induced 2BS cellular senescence was studied. The results showed that PGP was a linear polysaccharide containing alternating α-(1 → 3)- and β-(1 → 4)-galactopyranose units. Most of the sulfate groups are at C6 of the -(1 → 4)-α-D-Galp, and a small part of them are at C3 and C6. PGP pretreatment could decrease SA-β-gal-positive cells and prevent the formation of senescence-associated heterochromatic foci (SAHF) induced by H<sub>2</sub>O<sub>2</sub> in a dose-dependent manner. It is speculated that PGP may delay aging by downregulating the expression of p21 and p53 genes. The finding provides new insights into the beneficial role of <italic>G</italic>. <italic>lemaneiformis</italic> polysaccharide (GP) on retarding senescence process.</p>