AUTHOR=Wang Zheng , Wang Wenqian , Zhu Changlong , Gao Xiangdong , Chu Weihua 

TITLE=Evaluation of Antioxidative and Neuroprotective Activities of Total Flavonoids From Sea Buckthorn (Hippophae rhamnoides L.)

JOURNAL=Frontiers in Nutrition

VOLUME=9

YEAR=2022

URL=https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2022.861097

DOI=10.3389/fnut.2022.861097

ISSN=2296-861X

ABSTRACT=<p>The aim of this study was to investigate the antioxidative and neuroprotective activities of total flavonoids from sea buckthorn (<italic>Hippophae rhamnoides</italic> L.) (TFH). Results indicated that TFH possessed DPPH radicals, hydroxyl radicals and superoxide anions scavenging activities. The neuroprotective potential was assessed with acetylcholinesterase (AChE) and monoamine oxidase A (MAO-A). The inhibition rates of AChE and MAO-A by 50 μg/ml TFH were 75.85 and 51.22%, respectively. The <italic>in vivo</italic> antioxidative and neuroprotective potential of TFH were explored in <italic>Caenorhabditis elegans</italic>. In the longevity assay, TFH (50 μg/ml) significantly increased the lifespan of wild-type <italic>C. elegans</italic> (29.40%). In the hydrogen peroxide-induced oxidative stress challenge, the antioxidant capacity of TFH-treated wild-type <italic>C. elegans</italic> was significantly enhanced. The <italic>C. elegans</italic> mutant strain CL4176 was used to study the neuroprotective effect of TFH <italic>in vivo</italic>. Results showed that TFH significantly delayed paralysis in <italic>C. elegans</italic> CL4176. Our study suggested total flavonoids from sea buckthorn (<italic>Hippophae rhamnoides</italic> L.) had the potential as an antioxidative and neuroprotective agent to extend aging and treat neurodegenerative diseases.</p>