AUTHOR=Fang HaoHang , Zhuang ZhenXiao , Huang LuoDong , Zhao Wei , Niu Jin 

TITLE=Dietary Klebsormidium sp. Supplementation Improves Growth Performance, Antioxidant and Anti-Inflammatory Status, Metabolism, and Mid-Intestine Morphology of Litopenaeus Vannamei

JOURNAL=Frontiers in Nutrition

VOLUME=9

YEAR=2022

URL=https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2022.857351

DOI=10.3389/fnut.2022.857351

ISSN=2296-861X

ABSTRACT=<p>Filamentous microalga <italic>Klebsormidium</italic> sp. has huge potential to become a natural and healthy additive in aquatic feed since it contains various bioactive nutrients, such as linoleic acid (LA), carotenoids, and chlorophylls. Therefore, an eight-week feeding experiment was performed to evaluate the effects of dietary <italic>Klebsormidium</italic> sp. on the growth performance, antioxidant and anti-inflammatory status, metabolism, and mid-intestine morphology of <italic>Litopenaeus vannamei</italic>. Two isonitrogenous and isolipid diets supplemented with and without 5% <italic>Klebsormidium</italic> sp. were prepared. Results showed that <italic>L. vannamei</italic> fed with <italic>Klebsormidium</italic> sp. had better growth performance and feed utilization by optimizing mid-intestine morphology and improving the carbohydrate metabolism. In addition, <italic>Klebsormidium</italic> sp. also enhanced the antioxidant capacity of <italic>L. vannamei</italic> by downregulating antioxidant parameters (hepatopancreas T-SOD, hepatopancreas GSH-PX, hemolymph T-SOD, hemolymph MDA) and RNA expression levels of antioxidant genes (<italic>gsh-px</italic> and <italic>cat</italic>). Furthermore, the supplementations of dietary <italic>Klebsormidium</italic> sp. significantly improved hepatopancreas health by downregulating RNA expression levels of pro-inflammatory related genes (<italic>relish</italic> and <italic>rho</italic>). Therefore, a dose of 5% <italic>Klebsormidium</italic> sp. is recommended for the daily diet of <italic>L. vannamei</italic> to improve the growth performance, antioxidant and anti-inflammatory status, metabolism, and mid-intestine morphology of shrimp.</p>