AUTHOR=Deng Yongfei , Liu Han , Huang Qian , Tu Lingyun , Hu Lu , Zheng Bisheng , Sun Huaiqing , Lu Dengjun , Guo Chaowan , Zhou Lin 

TITLE=Mechanism of Longevity Extension of Caenorhabditis elegans Induced by Schizophyllum commune Fermented Supernatant With Added Radix Puerariae

JOURNAL=Frontiers in Nutrition

VOLUME=9

YEAR=2022

URL=https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2022.847064

DOI=10.3389/fnut.2022.847064

ISSN=2296-861X

ABSTRACT=<p><italic>Schizophyllum commune</italic> (<italic>S. commune</italic>) fermented supernatant with added Radix Puerariae (SC-RP) showed significant antioxidant activity in our previous work. However, the possible lifespan and healthspan extending the capacity of <italic>Caenorhabditis elegans</italic> (<italic>C. elegans</italic>) and the underlying mechanism were not illuminated. In this study, the effect of SC-RP on extending the lifespan and improving stress resistance of <italic>C. elegans</italic> were examined. Additionally, the underlying lifespan extending molecular mechanisms of SC-RP were explored. Treated with SC-RP at 10 μg/mL, the lifespan of <italic>C. elegans</italic> increased by 24.89% (<italic>P</italic> &lt; 0.01). Also, SC-RP prolonged the healthspan of the nematode, including reducing lipofuscin levels, improving mobility and enhancing resistance to oxidative stress and heat shock. Moreover, superoxide dismutase and catalase activities were increased for SC-RP treated <italic>C. elegans</italic>. Meantime the intracellular levels of thiobarbituric acid reactive substances (TBARS) and reactive oxygen species (ROS) were attenuated. Express levels of eight genes including <italic>daf-2, daf-16, sod-3, skn-1, gst-4, clk-1, age-1 and mev-1</italic> were analyzed by RT-PCR method for possible <italic>C. elegan</italic> anti-aging mechanisms of SC-RP. Expression levels of key genes <italic>daf-2, gst-4 and sod-3</italic> were up-regulated, while that of <italic>daf-16, skn-1</italic>, and <italic>clk-1</italic> were down-regulated. The results suggest that SC-RP could extend the lifespan and healthspan of <italic>C. elegans</italic> significantly, and the IIS pathway, SKN-1/Nrf2 pathway and mitochondrial metabolism pathway were primarily considered associated. Thus, SC-RP is a potential component to improve aging and aging-related symptoms as new functional materials.</p>