AUTHOR=Xu Yongjin , Zhong Fei , Zheng Xiaoqian , Lai Hsin-Yi , Wu Chunchun , Huang Cong 

TITLE=Disparity of Gut Microbiota Composition Among Elite Athletes and Young Adults With Different Physical Activity Independent of Dietary Status: A Matching Study

JOURNAL=Frontiers in Nutrition

VOLUME=9

YEAR=2022

URL=https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2022.843076

DOI=10.3389/fnut.2022.843076

ISSN=2296-861X

ABSTRACT=<sec><title>Objective</title><p>This study aimed to investigate the disparity of gut microbiota among elite athletes and young adults with different physical activity independent of dietary status.</p></sec><sec><title>Methods</title><p>In Hangzhou, China, an age and sex matching study was conducted between April and May 2021. A total of 66 Chinese young adults were recruited in this study and divided into an elite athlete group, physically active group, and physically inactive group. Fecal samples were collected to assess gut microbiota composition. Dietary status was measured using a food-frequency questionnaire. Comparisons in gut microbiota and blood biomarkers among three groups were analyzed by using the analysis of covariance.</p></sec><sec><title>Results</title><p>The findings depicted a tendency to form clusters for beta diversity among three groups, while no significant difference was observed in both alpha and beta diversity. In the multiple analysis model, by adjusting dietary status, a significantly higher abundance of <italic>Clostridiaceae</italic> (<italic>p</italic> = 0.029) and <italic>Megamonas_rupellensis</italic> (<italic>p</italic> = 0.087) was observed in elite athletes compared to that in general young adults. Furthermore, inflammation-related bacteria such as <italic>Bilophila</italic> (<italic>p</italic> = 0.011) and <italic>Faecalicoccus</italic> (<italic>p</italic> = 0.050) were enriched in physically inactive young adults compared to two other groups. Pearson's correlation analysis showed a positive association between <italic>Bilophila</italic> and circulating white body cell count (<italic>r</italic> = 0.332, <italic>p</italic> = 0.006) and its subtypes including neutrophils (<italic>r</italic> = 0.273, <italic>p</italic> = 0.027), and lymphocytes (<italic>r</italic> = 0.327, <italic>p</italic> = 0.007). <italic>Megamonas_rupellensis</italic> has been shown associated positively with serum lymphocytes levels (<italic>r</italic> = 0.268, <italic>p</italic> = 0.03). Although no significant differences were observed, the elite athletes tended to have lower levels of blood biomarkers of immunity within a normal range, which may reflect a better immune function.</p></sec><sec><title>Conclusion</title><p>This matching study indicated that physically inactive young adults are more likely to have a lower immune function and a higher abundance of pro-inflammatory gut bacteria than elite athletes and physically active young adults. Dietary status should be considered as an important factor that may affect the association of physical activity with immune function and gut microbiota.</p></sec>