AUTHOR=Wang Xiaoyu , Liu Guoqing , Xie Shouqi , Pan Lei , Tan Qingsong 

TITLE=Growth and Meat Quality of Grass Carp (Ctenopharyngodon idellus) Responded to Dietary Protein (Soybean Meal) Level Through the Muscle Metabolism and Gene Expression of Myosin Heavy Chains

JOURNAL=Frontiers in Nutrition

VOLUME=9

YEAR=2022

URL=https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2022.833924

DOI=10.3389/fnut.2022.833924

ISSN=2296-861X

ABSTRACT=<p>The aim of this study was to investigate the effect of dietary protein level (soybean meal) on growth performance, flesh quality of grass carp, and the related molecular mechanisms. The results showed that appropriate dietary protein levels improved the growth performance, hardness, and pH of muscle while decreasing muscle crude lipid content and cooking loss and altering the antioxidant capacity and metabolic enzymes activities. In addition, appropriate dietary protein promoted the gene expression of <italic>myhc-1, myhc</italic>-<italic>4, myf5, myod, myog</italic>, and <italic>fgf6a</italic>, whereas inhibited that of <italic>myhc-7, myhc</italic>-<italic>2, mrf4</italic>, and <italic>mstn</italic>. Transcriptome profiling of muscle revealed that the flesh quality-specific differences were related to tight junctions and intramuscular fat (IMF) accumulation. GSEA showed that fatty acid metabolism and oxidative phosphorylation were downregulated in SM5 compared with SM1. To conclude, appropriate protein levels improved the growth and flesh quality by regulating muscle antioxidant capacity and gene expression of <italic>myhc</italic>s and fat metabolism-related signaling molecules.</p>