AUTHOR=Hu Rui , Zhang Yong , Qian Weiyi , Leng Yan , Long Yan , Liu Xinjie , Li Jinping , Wan Xiangyuan , Wei Xun 

TITLE=Pediococcus acidilactici Promotes the Longevity of C. elegans by Regulating the Insulin/IGF-1 and JNK/MAPK Signaling, Fat Accumulation and Chloride Ion

JOURNAL=Frontiers in Nutrition

VOLUME=9

YEAR=2022

URL=https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2022.821685

DOI=10.3389/fnut.2022.821685

ISSN=2296-861X

ABSTRACT=<p>Probiotics are known to contribute to the anti-oxidation, immunoregulation, and aging delay. Here, we investigated the extension of lifespan by fermented pickles-origin <italic>Pediococcus acidilactici</italic> (PA) in <italic>Caenorhabditis elegans (C. elegans)</italic>, and found that PA promoted a significantly extended longevity of wild-type <italic>C. elegans</italic>. The further results revealed that PA regulated the longevity <italic>via</italic> promoting the insulin/IGF-1 signaling, JNK/MAPK signaling but not TOR signaling in <italic>C. elegans</italic>, and that PA reduced the reactive oxygen species (ROS) levels and modulated expression of genes involved in fatty acids uptake and lipolysis, thus reducing the fat accumulation in <italic>C. elegans</italic>. Moreover, this study identified the <italic>nrfl-1</italic> as the key regulator of the PA-mediated longevity, and the <italic>nrfl-1/daf-18</italic> signaling might be activated. Further, we highlighted the roles of one chloride ion exchanger gene <italic>sulp-6</italic> in the survival of <italic>C. elegans</italic> and other two chloride ion channel genes <italic>clh-1</italic> and <italic>clh-4</italic> in the prolonged lifespan by PA-feeding through the modulating expression of genes involved in inflammation. Therefore, these findings reveal the detailed and novel molecular mechanisms on the longevity of <italic>C. elegans</italic> promoted by PA.</p>