AUTHOR=Wang Xianshu , Yue Hao , Zhang Haonan , Wan Lei , Ji Shuxia , Geng Chong 

TITLE=Preventive Effects of Long-Term Intake of Plant Oils With Different Linoleic Acid/Alpha-Linolenic Acid Ratios on Acute Colitis Mouse Model

JOURNAL=Frontiers in Nutrition

VOLUME=9

YEAR=2022

URL=https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2022.788775

DOI=10.3389/fnut.2022.788775

ISSN=2296-861X

ABSTRACT=<sec><title>Objective</title><p>To investigate the preventive effects of plant oils with different linoleic acid/alpha-linolenic acid (LA/ALA) ratios against colitis symptoms, and dysbiosis of gut microbiota in acute colitis mouse model.</p></sec><sec><title>Methods</title><p>Sixty male C57BL/6 mice were assigned into six groups (<italic>n</italic> = 10): three groups were fed low-fat diets with low, medium, and high LA/ALA ratios; and three groups were fed with high-fat diets with low, medium, and high LA/ALA ratios. After 3 months of diet, the mice were exposed to dextran sodium sulfate solution to induce acute colitis. The severity of colitis was estimated by disease activity index (DAI) and histopathological examination. <italic>16S rRNA</italic> gene sequencing was used for the analysis of gut microbiota.</p></sec><sec><title>Results</title><p>Plant oils with a lower LA/ALA ratio showed higher alleviating effects on the symptoms of colitis, which were accompanied by the better prebiotic characteristics manifested as effectively inhibiting the abnormal expansion of phylum <italic>Proteobacteria</italic> and genus <italic>Escherichia-Shigella</italic> in the gut microbiota of colitis mouse models.</p></sec><sec><title>Conclusion</title><p>A potential IBD prevention strategy of reducing the LA/ALA ratio in the daily consumed plant oils was proposed in this study. Furthermore, based on the optimized LA/ALA ratio, this preventive effect might not be weakened by the high intake of plant oils.</p></sec>