AUTHOR=Zhang Zuo-fa , Song Ting-ting , Chen Jian-fei , Lv Guo-ying 

TITLE=Recovery of a hypolipidemic polysaccharide from artificially cultivated Sanghuangporus vaninii with an effective method

JOURNAL=Frontiers in Nutrition

VOLUME=9

YEAR=2023

URL=https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2022.1095556

DOI=10.3389/fnut.2022.1095556

ISSN=2296-861X

ABSTRACT=<p>In this study, an effective method was developed to extract the polysaccharide from <italic>Sanghuangporus vaninii</italic> (PFSV) by destroying the cell wall. Box-Behnken design was employed to determine the optimal processing conditions as follows: processing temperature (80°C), processing time (0.81 h) and amount of HCl (1.5 ml). Under these conditions, the yield of PFSV reached 5.94 ± 0.16%. The purified polysaccharide (PFSV-2) was found to be a hetero-polysaccharide with an average molecular weight of 20.377 kDa. The backbone of PFSV-2 was composed of an →6)-<italic>α</italic>-Gal<italic>p</italic>-(1→ and →2,6)-<italic>β</italic>-Man<italic>p</italic>-(1→ and →2)-<italic>α</italic>-Fuc<italic>p</italic>-(1→ and was branched of t-<italic>α</italic>-Man<italic>p</italic>-(1→ at position 2 of residue B. PFSV-2 showed hypolipidemic activity by decreasing lipid accumulation and the levels of total cholesterol and triglycerides in zebrafish larvae. Furthermore, PFSV-2 downregulated the <italic>pparg, fasn</italic>, and <italic>HMGCRb</italic> genes and upregulated the <italic>pparab</italic> and <italic>acaca</italic> genes. These findings suggested PFSV-2 may be a promising candidate in lipid regulation therapy.</p>