AUTHOR=Wang Chen , Zhang Xiaoming , Liu Minghui , Qin Shengtang , He Chengrong , Liu Yingnan , Huai Jing , Zhang Qidi , Wei Yumei , Yang Huixia 

TITLE=Irisin participates in the beneficial effects of exercise in preventing gestational diabetes mellitus in overweight and obese pregnant women and a mouse model

JOURNAL=Frontiers in Nutrition

VOLUME=9

YEAR=2023

URL=https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2022.1034443

DOI=10.3389/fnut.2022.1034443

ISSN=2296-861X

ABSTRACT=<sec><title>Objective</title><p>We aimed to explore whether irisin participates in the beneficial effects of exercise in preventing gestational diabetes mellitus (GDM) in overweight and obese pregnant women.</p></sec><sec><title>Study design</title><p>Sixty overweight and obese pregnant women each in the exercise and control groups were randomly selected from our previous randomized controlled trial. Eighteen obese model mice were generated and divided into exercise and control groups in which body weight, abdominal circumference, anal temperature, glucose tolerance test, and insulin tolerance test were recorded. The plasma irisin level, the expression of <italic>PGC-1</italic>α<italic>/FNDC5</italic> and brown (<italic>UCP1</italic>) and beige adipose (<italic>CD137</italic>, <italic>TMEM26</italic>, and <italic>TBX-1</italic>) marker genes were detected in muscle and adipose tissue.</p></sec><sec><title>Results</title><p>In the human study, women in the exercise group had a significantly higher irisin level and lower insulin resistance level than those in the control group. Enhanced expression of beige adipose tissue marker genes (<italic>CD137</italic>, <italic>TMEM26</italic>, and <italic>TBX-1</italic>) in omental adipose tissue and the <italic>CD137</italic> gene in subcutaneous adipose tissue were found in the exercise group compared to the control group. In a mouse model, body weight and abdominal circumference were decreased, while glucose homeostasis and insulin sensitivity were significantly improved, and anal temperature was elevated after exercise intervention. A significantly higher level of irisin was revealed in the exercise group after undergoing exercise treatment. The expression of the beige adipose marker genes <italic>CD137</italic> and <italic>TBX-1</italic> was significantly higher in the exercise group than in the control group in posterior subcutaneous adipose tissue from the inguinal area and interscapular adipose tissue respectively.</p></sec><sec><title>Conclusion</title><p>Our observations show that regular exercise during pregnancy can increase irisin levels, promote white fat beiging/browning, improve glucose homeostasis and enhance body energy expenditure, which may be one of the mechanisms by which exercise prevents GDM.</p></sec>