AUTHOR=Li Chong , Fan Yang , Li Shuang , Zhou Xianrong , Park Kun-Young , Zhao Xin , Liu Huazhi 

TITLE=Antioxidant Effect of Soymilk Fermented by Lactobacillus plantarum HFY01 on D-Galactose-Induced Premature Aging Mouse Model

JOURNAL=Frontiers in Nutrition

VOLUME=8

YEAR=2021

URL=https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2021.667643

DOI=10.3389/fnut.2021.667643

ISSN=2296-861X

ABSTRACT=<p>The antioxidant effect of soymilk fermented by <italic>Lactobacillus plantarum</italic> HFY01 (screened from yak yogurt) was investigated on mice with premature aging induced by D-galactose. <italic>In vitro</italic> antioxidant results showed that <italic>L. plantarum</italic> HFY01-fermented soymilk (LP-HFY01-DR) had better ability to scavenge the free radicals 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) diammonium salt (ABTS) than unfermented soymilk and <italic>Lactobacillus bulgaricus</italic>-fermented soymilk. Histopathological observation showed that LP-HFY01-DR could protect the skin, spleen and liver, reduce oxidative damage and inflammation. Biochemical results showed that LP-HFY01-DR could effectively upregulate glutathione (<italic>GSH</italic>), catalase (<italic>CAT</italic>), superoxide dismutase (<italic>SOD</italic>), and glutathione peroxidase (<italic>GSH-Px</italic>) levels and decrease malondialdehyde (<italic>MDA</italic>) content in the liver, brain, and serum. Real-time quantitative reverse transcription polymerase chain reaction further showed that LP-HFY01-DR could promote the relative expression levels of the genes encoding for cuprozinc superoxide dismutase (<italic>Cu/Zn-SOD, SOD1</italic>), manganese superoxide dismutase (<italic>Mn-SOD, SOD2</italic>), <italic>CAT, GSH</italic>, and <italic>GSH-Px</italic> in the liver, spleen, and skin. High-performance liquid chromatography results revealed daidzin, glycitin, genistin, daidzein, glycitein, and genistein in LP-HFY01-DR. In conclusion, LP-HFY01-DR could improve the antioxidant capacity in mice with premature aging induced by D-galactose.</p>