AUTHOR=Correia Daniela M. , Dias Luís G. , Veloso Ana C. A. , Dias Teresa , Rocha Isabel , Rodrigues Lígia R. , Peres António M. 

TITLE=Dietary Sugars Analysis: Quantification of Fructooligossacharides during Fermentation by HPLC-RI Method

JOURNAL=Frontiers in Nutrition

VOLUME=1

YEAR=2014

URL=https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2014.00011

DOI=10.3389/fnut.2014.00011

ISSN=2296-861X

ABSTRACT=<p>In this work, a simple chromatographic method is proposed and in-house validated for the quantification of total and individual fructooligossacharides (e.g., 1-kestose, nystose, and 1<sup>F</sup>-fructofuranosylnystose). It was shown that a high-performance liquid chromatography with refractive index detector could be used to monitor the dynamic of fructooligossacharides production via sucrose fermentation using <italic>Aspergillus aculeatus</italic>. This analytical technique may be easily implemented at laboratorial or industrial scale for fructooligossacharides mass-production monitoring allowing also controlling the main substrate (sucrose) and the secondary by-products (glucose and fructose). The proposed chromatographic method had a satisfactory intra- and inter-day variability (in general, with a relative standard deviation lower than 5%), high sensitivity for each sugar (usually, with a relative error lower than 5%), and low detection (lower than 0.06 ± 0.04 g/L) and quantification (lower than 0.2 ± 0.1 g/L) limits. The correct quantification of fructooligossacharides in fermentative media may allow a more precise nutritional formulation of new functional foods, since it is reported that different fructooligossacharides exhibit different biological activities and effects.</p>