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ORIGINAL RESEARCH article

Front. Neurosci.
Sec. Neuropharmacology
Volume 18 - 2024 | doi: 10.3389/fnins.2024.1491343

Annao Pingchong decoction attenuates oxidative stress and neuronal apoptosis following intracerebral hemorrhage via RAGE-NOX2/4 axis

Provisionally accepted
Xu Wang Xu Wang 1Xiaoyuan Lin Xiaoyuan Lin 1*Zilin Chen Zilin Chen 2Ping hong Long Ping hong Long 1旭晴 周 旭晴 周 1Shihui Lei Shihui Lei 1*Jian Liu Jian Liu 1*Huan Dong Huan Dong 1*Fang Liu Fang Liu 1*Hua Hu Hua Hu 3*Chun Guo Chun Guo 1*
  • 1 Experiment Center of Medical Innovation, The First Hospital of Hunan University of Chinese Medicine, Changsha, Anhui Province, China
  • 2 Department of Pediatrics, Guang’anmen Hospital, China Academy of Traditional Chinese Medicine, Beijing, China
  • 3 Department of Neurology, The First Hospital of Hunan University of Chinese Medicine, Changsha, Anhui Province, China

The final, formatted version of the article will be published soon.

    Background: Intracerebral hemorrhage (ICH) is a severe condition associated with high mortality and disability rates. Oxidative stress plays a critical role in the development of secondary brain injury (SBI) following ICH. Previous research has demonstrated that Annao Pingchong decoction (ANPCD) treatment for ICH has antioxidant effects, but the exact mechanism is not yet fully understood.Objective: This study aimed to investigate the neuroprotective effects of ANPCD on oxidative stress and neuronal apoptosis after ICH by targeting the receptor for advanced glycation end products (RAGE)-NADPH oxidase (NOX) 2/4 signaling axis.The research involved the creation of rat ICH models, the mNSS assay to assess neurological function, Nissl staining to evaluate neuronal damage, and biochemical assays to measure oxidative and antioxidant levels. The expression of RAGE-NOX2/4 axis proteins was analyzed using western blotting and immunofluorescence, while neuronal apoptosis was assessed with TUNEL staining. Furthermore, after performing quality control of drug-containing serum using UPLC-MS/MS, we employed an in vitro model of heme-induced injury in rat cortical neurons to investigate the neuroprotective mechanisms of ANPCD utilizing RAGE inhibitors.The findings indicated that ANPCD improved neurological deficits, reduced neuronal damage, decreased ROS and MDA levels, and increased the activities enzymatic activities of SOD, CAT, GSH and GPX. Additionally, it suppressed the RAGE-NOX2/4 signaling axis and neuronal apoptosis.ANPCD exhibits neuroprotective effects by inhibiting the RAGE-NOX2/4 signaling axis, thereby alleviating neuronal oxidative stress and apoptosis following ICH.

    Keywords: Annao Pingchong decoction, intracerebral hemorrhage, NOX2/4, Rage, oxidative stress ICH, Intracerebral hemorrhage, SBI, secondary brain injury, ANPCD, Annao Pingchong decoction, RAGE, receptor for advanced glycation end products

    Received: 04 Sep 2024; Accepted: 03 Dec 2024.

    Copyright: © 2024 Wang, Lin, Chen, Long, 周, Lei, Liu, Dong, Liu, Hu and Guo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Xiaoyuan Lin, Experiment Center of Medical Innovation, The First Hospital of Hunan University of Chinese Medicine, Changsha, Anhui Province, China
    Shihui Lei, Experiment Center of Medical Innovation, The First Hospital of Hunan University of Chinese Medicine, Changsha, Anhui Province, China
    Jian Liu, Experiment Center of Medical Innovation, The First Hospital of Hunan University of Chinese Medicine, Changsha, Anhui Province, China
    Huan Dong, Experiment Center of Medical Innovation, The First Hospital of Hunan University of Chinese Medicine, Changsha, Anhui Province, China
    Fang Liu, Experiment Center of Medical Innovation, The First Hospital of Hunan University of Chinese Medicine, Changsha, Anhui Province, China
    Hua Hu, Department of Neurology, The First Hospital of Hunan University of Chinese Medicine, Changsha, Anhui Province, China
    Chun Guo, Experiment Center of Medical Innovation, The First Hospital of Hunan University of Chinese Medicine, Changsha, Anhui Province, China

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