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ORIGINAL RESEARCH article

Front. Neurosci.
Sec. Neuropharmacology
Volume 18 - 2024 | doi: 10.3389/fnins.2024.1485873

Dopaminergic Modulation of Propofol-Induced Activation in VLPO Neurons: The Role of D1 Receptors in Sleep-Promoting Neural Circuits

Provisionally accepted
Kun Qian Kun Qian 1Yu Zhang Yu Zhang 2Yang Liu Yang Liu 2Wu Si Si Wu Si Si 2Zikun Duan Zikun Duan 2Jianhao Liao Jianhao Liao 2Wei Luo Wei Luo 2Mo Zhou Mo Zhou 2Xuejiao Dou Xuejiao Dou 1Xingkui Liu Xingkui Liu 2Tian Yu Tian Yu 2*
  • 1 Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou Province, China
  • 2 Zunyi Medical University, Zunyi, China

The final, formatted version of the article will be published soon.

    Background: The ventrolateral preoptic nucleus (VLPO) is a crucial regulator of sleep, and its neurons are implicated in both sleep-wake regulation and anesthesia-induced loss of consciousness. Propofol (PRO), a widely used intravenous anesthetic, modulates the activity of VLPO neurons, but the underlying mechanisms, particularly the role of dopaminergic receptors, remain unclear. Objective: This study aimed to investigate the effects of PRO on NA (-) neurons in the VLPO and to determine the involvement of D1 and D2 dopaminergic receptors in mediating these effects. Methods: Using in vitro patch-clamp techniques, we identified and characterized NA (-) and NA (+) neurons in the VLPO based on their morphological, pharmacological, and electrophysiological properties. We assessed the effects of PRO on spontaneous excitatory postsynaptic currents (sEPSCs) and inhibitory postsynaptic currents (sIPSCs) in NA (-) neurons, both in the presence and absence of dopaminergic receptor modulators. Results: PRO significantly increased the firing frequency of NA (-) neurons while decreasing the firing frequency of NA (+) neurons. This activation of NA (-) neurons was mediated through GABA_A receptors, as evidenced by the increased frequency of sEPSCs and altered sIPSCs dynamics. Dopamine (DA) attenuated the PRO-induced increase in sEPSCs frequency and suppression of sIPSCs frequency in NA (-) neurons via D1 receptors, but not D2 receptors. Blocking D1 receptors with SCH23390 reversed the effects of DA on PRO-induced changes, while D2 receptor antagonist sulpiride had minimal impact. Conclusion: Our findings demonstrate that PRO excites sleep-promoting NA (-) neurons in the VLPO, primarily through GABA_A receptors, with dopaminergic modulation occurring via D1 receptors. These results provide new insights into the neural mechanisms underlying general anesthesia and highlight the potential role of dopaminergic signaling in modulating anesthetic effects on sleep-related neural circuits.

    Keywords: Ventrolateral preoptic nucleus, Propofol, Spontaneous excitatory postsynaptic currents, Spontaneous inhibitory postsynaptic currents, D1 dopaminergic receptors

    Received: 25 Aug 2024; Accepted: 24 Dec 2024.

    Copyright: © 2024 Qian, Zhang, Liu, Si, Duan, Liao, Luo, Zhou, Dou, Liu and Yu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Tian Yu, Zunyi Medical University, Zunyi, China

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