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ORIGINAL RESEARCH article

Front. Neurosci.
Sec. Neuropharmacology
Volume 18 - 2024 | doi: 10.3389/fnins.2024.1450072
This article is part of the Research Topic Plant and Fungal Extracts and Metabolites in Neurotherapy: Exploring Their Pharmacology and Potential Clinical Uses View all articles

Topical application of daphnetin hydrogel for traumatic brain injury

Provisionally accepted
Yuanhao Ma Yuanhao Ma 1Yu Liu Yu Liu 2Jianqiang Guo Jianqiang Guo 2Zhongjun Chen Zhongjun Chen 2Zongren Zhao Zongren Zhao 2*Jinyu Zheng Jinyu Zheng 2,3*
  • 1 Huzhou Central Hospital, Huzhou, China
  • 2 Huai'an Second People's Hospital, Huaian, China
  • 3 Department of Neurosurgery, Huai'an Second People's Hospital, Huaian city, China

The final, formatted version of the article will be published soon.

    Background: Traumatic brain injury (TBI) causes neuronal cell damage and dysfunction. According to previous studies, daphnetin (Dap) has a protective effect in neurological injury. However, the in vivo bioavailability of daphnetin is not high. The purpose of this study was to determine whether administering daphnetin directly into the site of injury via a hydrogel drug carrier could improve its therapeutic impact. Methods: Tripolycerol monostearates / daphnetin (TM/Dap) hydrogels were prepared and characterised using water bath heating, scanning electron microscopy (SEM) and small animal in vivo imaging techniques. The TBI model was established using the Feeney free fall impact method. Using the Morris water maze test, the mNSS neurological deficit rating scale, haematoxylin-eosin staining, and liver and kidney function tests, the therapeutic benefit of TM/Dap and its toxic side effects were assessed. The therapeutic effects of TM/Dap were further investigated using wet and dry gravimetric methods, Evans blue staining, protein immunoblotting, immunofluorescence staining techniques and ELISA. Results: The efficacy of the TM/Dap hydrogel in gradually releasing daphnetin in the context of traumatic brain damage was shown by both in vitro and in vivo tests. Behavioural experiments showed that the learning and spatial memory abilities of TM/Dap hydrogel treated mice were significantly improved in the water maze experiment. And TM/Dap hydrogel has high biosafety for organisms. The results of the therapeutic mechanism of action showed that TM/Dap hydrogel showed more significant efficacy in reducing the neuroinflammatory response caused by TNF-α, IL-6 and other factors, as well as promoting the recovery of post-traumatic neurological function. Conclusions: The use of hydrogel as a drug carrier for daphnetin showed more significant efficacy in reducing neuroinflammatory response, protecting nerve tissue and promoting post-traumatic neurological recovery compared with traditional drug delivery methods.

    Keywords: Daphnetin, Hydrogel, Traumatic Brain Injury, Drug carrier, bioavailability

    Received: 16 Jun 2024; Accepted: 29 Jul 2024.

    Copyright: © 2024 Ma, Liu, Guo, Chen, Zhao and Zheng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Zongren Zhao, Huai'an Second People's Hospital, Huaian, 223002, China
    Jinyu Zheng, Department of Neurosurgery, Huai'an Second People's Hospital, Huaian city, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.