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REVIEW article
Front. Neurosci.
Sec. Translational Neuroscience
Volume 18 - 2024 |
doi: 10.3389/fnins.2024.1443438
This article is part of the Research Topic Immune Therapies in Neurological Disorders View all articles
Uncover dual roles of peripheral immune cells and connections to brain cells in stroke and post-stroke stage by single cell sequencing
Provisionally accepted- 1 Southern University of Science and Technology, Shenzhen, China
- 2 Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences (CAS), Shenzhen, Guangdong Province, China
Ischemic stroke is a cerebrovascular disease affecting the blood vessels and blood supply to the brain, which has become the second leading cause of death throughout the world. Studies suggested that immune cells play a dual role during the stroke inflammation and recovery phase, but their in-depth investigations of specific cell subtypes along with their differentiation trajectories are yet to be disentangled. In this review, we highlight the application of single-cell RNA sequencing (scRNA-seq) on unbiased identification of cell heterogeneity in brain and peripheral blood mononuclear cells (PBMCs) during and after stroke, aiming to unravel the phenotypic landscape of cells with different roles in stroke. Specifically, we provide an overview of roles, cell surface markers, immune cell-released cytokines and intercellular interactions found by different technologies in major immune cells during and after stroke, and summarised the connection of immune cells between peripheral blood and brain via differentiation trajectories. Through the above reviews, we synthesise the application of scRNA-seq on the combination of PBMCs and brain tissue at higher sampling frequency to unveil the dual role of peripheral immune cells, which could aid the development of new treatment strategies of ischemic stroke.p
Keywords: Stroke, post-stroke, scRNA seq, PBMC, Dual role
Received: 04 Jun 2024; Accepted: 24 Oct 2024.
Copyright: © 2024 Xu, Yang and Zheng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Lifang Zheng, Southern University of Science and Technology, Shenzhen, China
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