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SYSTEMATIC REVIEW article

Front. Neurosci.
Sec. Neurodegeneration
Volume 18 - 2024 | doi: 10.3389/fnins.2024.1432102

Characterizing the Adult Zebrafish Model of Parkinson's Disease: A Systematic Review of Dynamic Changes in Behavior and Physiology Post-MPTP Administration

Provisionally accepted
Khairiah Razali Khairiah Razali 1Jaya Kumar Jaya Kumar 2*Wael M. Mohamed Wael M. Mohamed 3*
  • 1 Department of Basic Medical Sciences, Kulliyyah of Medicine, International Islamic University Malaysia, Kuantan, Pahang Darul Makmur, Malaysia
  • 2 Department of Physiology, Universiti Kebangsaan Malaysia Medical Centre, Bandar Tun Razak, Kuala Lumpur, 56000, Malaysia
  • 3 Department of Clinical Pharmacology, Faculty of Medicine, Menoufia University, Shebin El-Kom, Monufia, Egypt

The final, formatted version of the article will be published soon.

    Introduction: Adult zebrafish are increasingly used in Parkinson's disease (PD) research due to their well-characterized dopaminergic system. Among the toxin-based models, the neurotoxin 1-methyl-4phenyl-1,2,3,6-tetrahydropyridine (MPTP) is widely utilized to induce parkinsonism in adult zebrafish. Therefore, this review presents an overview of the procedures and the dynamic changes in behavior and physiology observed in the adult zebrafish PD model following a single intraperitoneal injection of MPTP. Methods: A systematic literature search in the PubMed and Google Scholar databases was conducted to identify relevant articles. Of the 165 articles identified, 9 were included in this review. These chosen articles are original works published before March 2024, all of which utilized adult zebrafish induced with MPTP as the model for PD. Other articles were excluded based on factors such as limited relevance, utilization of zebrafish embryos or larvae instead of adults, and variations in MPTP deliveries. Results: Studies indicated that the ideal model entails the utilization of mixed gender zebrafish aged between four to six months from the wild-type strain. The acceptable MPTP doses ranges between 20 µg/g (lowest) and 225 µg/g (highest) and doses above 292 µg/g are lethal. Furthermore, noticeable parkinsonian symptoms appear one day after administration and persist for more than one week. Discussion: Mitochondrial dysfunction precedes dopaminergic neurodegeneration within this experimental regime. A single administration of MPTP effectively induces PD in adult zebrafish. This study aids in crafting the adult zebrafish PD model, outlining the progressive behavioral and physiological changes ensuing from MPTP administration.

    Keywords: Parkinson's disease, Zebrafish, MPTP, Intraperitoneal injection, Mitochondrial dysfunction

    Received: 13 May 2024; Accepted: 13 Aug 2024.

    Copyright: © 2024 Razali, Kumar and Mohamed. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Jaya Kumar, Department of Physiology, Universiti Kebangsaan Malaysia Medical Centre, Bandar Tun Razak, Kuala Lumpur, 56000, Malaysia
    Wael M. Mohamed, Department of Clinical Pharmacology, Faculty of Medicine, Menoufia University, Shebin El-Kom, Monufia, Egypt

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.