Skip to main content

CORRECTION article

Front. Neurosci., 28 November 2023
Sec. Neurodevelopment
Volume 17 - 2023 | https://doi.org/10.3389/fnins.2023.1338853

Corrigendum: PlexinD1 signaling controls domain-specific dendritic development in newborn neurons in the postnatal olfactory bulb

This article is a correction to:

  1. PlexinD1 signaling controls domain-specific dendritic development in newborn neurons in the postnatal olfactory bulb

    1. Read original article
\r\nMasato Sawada,&#x;Masato Sawada1,2†Ayato HamaguchiAyato Hamaguchi1Naomichi ManoNaomichi Mano1Yutaka Yoshida,,Yutaka Yoshida3,4,5Akiyoshi UemuraAkiyoshi Uemura6Kazunobu Sawamoto, &#x;Kazunobu Sawamoto1,2*†
  • 1Department of Developmental and Regenerative Neurobiology, Institute of Brain Science, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan
  • 2Division of Neural Development and Regeneration, National Institute of Physiological Sciences, Okazaki, Japan
  • 3Burke Neurological Institute, White Plains, NY, United States
  • 4Brain and Mind Research Institute, Weill Cornell Medicine, New York, NY, United States
  • 5Neural Circuit Unit, Okinawa Institute of Science and Technology Graduate University, Okinawa, Japan
  • 6Department of Retinal Vascular Biology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan

A corrigendum on
PlexinD1 signaling controls domain-specific dendritic development in newborn neurons in the postnatal olfactory bulb

by Sawada, M., Hamaguchi, A., Mano, N., Yoshida, Y., Uemura, A., and Sawamoto, K. (2023). Front. Neurosci. 17:1143130. doi: 10.3389/fnins.2023.1143130

In the published article, there was an error in Figures 2 and 3 as published. In Figures 2E and 3F, “Wilt-type” is a typographical error of “Wild-type”. The corrected Figures 2 and 3 appear below.

FIGURE 2
www.frontiersin.org

Figure 2. PlexinD1's RBD is involved in the PlexinD1-mediated suppression of lateral dendrite formation in granule cells in the postnatal OB. (A) Molecular structure of PlexinD1. (B) Experimental scheme of PlexinD1- and PlexinD1ΔRBD-overexpressing neuronal culture. (C) Representative images of Venus+ (green) Dcx+ (white) cultured control, PlexinD1-overexpressing, and PlexinD1ΔRBD-overexpressing neurons. Red indicates PlexinD1. (D) Relative PlexinD1 intensity in the infected neurons (control, n = 49 cells; PlexinD1, n = 38 cells; PlexinD1ΔRBD, n = 30 cells; three independent experiments). (E) Experimental scheme for PlexinD1 overexpression in vivo. (F–H) Representative projection images of Venus+ control (F), PlexinD1-overexpressing (G), and PlexinD1ΔRBD-overexpressing (H) granule cells at 10 dpi. (I) Proportions of lateral dendrite-bearing granule cells at 10 dpi (control, n = 2,217 cells from 5 mice; PlexinD1, n = 4,204 cells from 5 mice; PlexinD1ΔRBD, n = 1,641 cells from 5 mice). Pink arrows indicate dendritic branches in the proximal domain of the apical dendrite. GCL, granule cell layer; RBD, Rho binding domain. *p < 0.05, ***p < 0.005. Scale bars: (C), 10 μm; (F–H), 20 μm. Bars indicate mean ± SEM.

FIGURE 3
www.frontiersin.org

Figure 3. RhoJ is expressed in migrating and differentiating granule cells in the postnatal OB and involved in the suppression of their dendritic branching in the proximal domain of the apical dendrite. (A) Representative images of the coronal V-SVZ sections in RhoJ+/GFP mice stained for GFP (green), Dcx (red), and CD31 (cyan). Nuclei were stained with Hoechst 33342 (Blue). (B–D) Representative images of the coronal OB sections in RhoJ+/GFP mice stained for GFP (green), Dcx (red), and NeuN (cyan). Boxed area in (B) was enlarged in (C) and (D). White arrows, yellow arrowheads, and cyan arrowheads (C) and (D) indicate GFP + Dcx + NeuN-, GFP + Dcx + NeuN+, and GFP + Dcx-NeuN+ granule cells, respectively. (E) Proportions of RhoJ+/GFP-positive cells in the OB (n = 3 mice; 144 cells analyzed). (F) Experimental scheme for RhoJ overexpression experiment. (G) Representative dendritic tracings of control (n = 32 cells from 4 mice) and RhoJ-overexpressing (n = 36 cells from 8 mice) granule cells at 10 day-post injection (dpi). (H–J) Dendritic branch numbers of distal [(H); control, n = 32 cells from 4 mice; RhoJ, n = 36 cells from 8 mice], proximal [(I); control, n = 231 cells from 4 mice; RhoJ, n = 67 cells from 8 mice], and basal [(J); control, n = 32 cells from 4 mice; RhoJ, n = 36 cells from 8 mice] domains in control and RhoJ-overexpressing granule cells at 10 dpi. (K) Experimental scheme for RhoJ loss-of-function experiment. (L) Representative dendritic tracings of control (n = 43 cells from 3 mice), RhoJ-KO (n = 47 cells from 3 mice), and PlexinD1-overexpressing RhoJ-KO (n = 24 cells from 4 mice) granule cells at 14 dpi. (M–O) Dendritic branch numbers of distal [(M); control, n = 43 cells from 3 mice; RhoJ-KO, n = 47 cells from 3 mice; RhoJ-KO + PlexinD1, n = 24 cells from 4 mice], proximal [(N); control, n = 137 cells from 3 mice; RhoJ-KO, n = 140 cells from 3 mice; RhoJ-KO + PlexinD1, n = 128 cells from 4 mice], and basal [(O); control, n = 43 cells from 3 mice; RhoJ-KO, n = 47 cells from 3 mice; RhoJ-KO + PlexinD1, n = 24 cells from 4 mice] domains in control, RhoJ-KO, and PlexinD1-overexpressing RhoJ-KO granule cells at 14 dpi. (P) Mechanism of dendritic branching in the proximal domain of the apical dendrite in granule cells in the postnatal OB. Pink arrows indicate dendritic branches in the proximal domain of the apical dendrite. V-SVZ, ventricular-subventricular zone; LV, lateral ventricle; RMS, rostral migratory stream; GCL, granule cell layer. *p < 0.05, ***p < 0.005. Scale bars: (A), (B), (G), (L), 20 μm; (C), (D), 10 μm. Bars indicate mean ± SEM.

The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.

Publisher's note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Keywords: postnatal neurogenesis, ventricular-subventricular zone, olfactory bulb, newborn neurons, dendrites, PlexinD1, RhoJ

Citation: Sawada M, Hamaguchi A, Mano N, Yoshida Y, Uemura A and Sawamoto K (2023) Corrigendum: PlexinD1 signaling controls domain-specific dendritic development in newborn neurons in the postnatal olfactory bulb. Front. Neurosci. 17:1338853. doi: 10.3389/fnins.2023.1338853

Received: 15 November 2023; Accepted: 16 November 2023;
Published: 28 November 2023.

Approved by:

Frontiers Editorial Office, Frontiers Media SA, Switzerland

Copyright © 2023 Sawada, Hamaguchi, Mano, Yoshida, Uemura and Sawamoto. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Kazunobu Sawamoto, c2F3YW1vdG8mI3gwMDA0MDttZWQubmFnb3lhLWN1LmFjLmpw

†ORCID: Masato Sawada orcid.org/0000-0002-8694-8526
Kazunobu Sawamoto orcid.org/0000-0003-1984-5129

Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.