AUTHOR=Lin Qihuan , Chen Lanmei , Zheng Hongyi , Tan Hui , Zhang Gengbiao , Zheng Wenbin 

TITLE=Imaging of nerve injury in neonatal acute bilirubin encephalopathy using 1H-MRS and Glu-CEST techniques

JOURNAL=Frontiers in Neuroscience

VOLUME=17

YEAR=2023

URL=https://www.frontiersin.org/journals/neuroscience/articles/10.3389/fnins.2023.1110349

DOI=10.3389/fnins.2023.1110349

ISSN=1662-453X

ABSTRACT=<sec><title>Objectives</title><p>To investigate the significance of proton magnetic resonance spectroscopy (<sup>1</sup>H-MRS) and glutamate chemical exchange saturation transfer (Glu-CEST) techniques in assessing the condition and prognosis of acute bilirubin encephalopathy patients and to understand the mechanism of nerve injury in this disease.</p></sec><sec><title>Materials and methods</title><p>From September 2019 to February 2021, 31 neonates with acute bilirubin encephalopathy and 16 healthy neonates were enrolled in this study. All the quantitative results of <sup>1</sup>H-MRS, Glu-CEST, and conventional magnetic resonance imaging (MRI) of all neonates were analyzed. The associations between statistically significant indicators of imaging and developmental quotients (DQ) were analyzed.</p></sec><sec><title>Results</title><p>The 31 cases were assigned to the mild subgroup (<italic>n</italic> = 21) and moderate and severe subgroup (<italic>n</italic> = 10) according to the bilirubin-induced neurologic dysfunction (BIND) scores. The case group had elevated Cho and GABA absolute concentrations compared to the normal control group (all <italic>p</italic> &lt; 0.05). Compared with the normal control group, the absolute concentration of GABA of the moderate and severe subgroup was significantly larger (<italic>p</italic> &lt; 0.05). Compared with the normal control group, the Glu-CEST% values in the left basal ganglia, right thalamus, left frontal cortex and bilateral medial geniculate body of the case group was significantly larger (all <italic>p</italic> &lt; 0.05). The moderate and severe subgroup had higher Glu-CEST% values in the left basal ganglia, right thalamus, and bilateral medial geniculate body than the normal control group (all <italic>p</italic> &lt; 0.05). A negative association was revealed between the DQ scores and the Glu-CEST% values in the left basal ganglia (<italic>r</italic> = −0.888, <italic>p</italic> &lt; 0.05).</p></sec><sec><title>Conclusion</title><p>The combination of <sup>1</sup>H-MRS and Glu-CEST techniques can monitor the intracerebral metabolite level of acute bilirubin encephalopathy and evaluate the illness severity.</p></sec>