AUTHOR=Sinefeld David , Xia Fei , Wang Mengran , Wang Tianyu , Wu Chunyan , Yang Xusan , Paudel Hari P. , Ouzounov Dimitre G. , Bifano Thomas G. , Xu Chris TITLE=Three-Photon Adaptive Optics for Mouse Brain Imaging JOURNAL=Frontiers in Neuroscience VOLUME=16 YEAR=2022 URL=https://www.frontiersin.org/journals/neuroscience/articles/10.3389/fnins.2022.880859 DOI=10.3389/fnins.2022.880859 ISSN=1662-453X ABSTRACT=
Three-photon microscopy (3PM) was shown to allow deeper imaging than two-photon microscopy (2PM) in scattering biological tissues, such as the mouse brain, since the longer excitation wavelength reduces tissue scattering and the higher-order non-linear excitation suppresses out-of-focus background fluorescence. Imaging depth and resolution can further be improved by aberration correction using adaptive optics (AO) techniques where a spatial light modulator (SLM) is used to correct wavefront aberrations. Here, we present and analyze a 3PM AO system for