AUTHOR=Carta Giacomo , Seregni Agnese , Casamassima Andrea , Galli Manuela , Geuna Stefano , Pagliaro Pasquale , Zago Matteo 

TITLE=Validation and Reliability of a Novel Vagus Nerve Neurodynamic Test and Its Effects on Heart Rate in Healthy Subjects: Little Differences Between Sexes

JOURNAL=Frontiers in Neuroscience

VOLUME=15

YEAR=2021

URL=https://www.frontiersin.org/journals/neuroscience/articles/10.3389/fnins.2021.698470

DOI=10.3389/fnins.2021.698470

ISSN=1662-453X

ABSTRACT=<sec><title>Background</title><p>The vagus nerve (VN), also called the pneumogastric nerve, connects the brainstem to organs contained in the chest and abdomen. Physiologically, VN stimulation can rapidly affect cardiac activity and heart rate (HR). VN neuropathy can increase the risk of arrhythmias and sudden death. Therefore, a selective test of VN function may be very useful. Since peripheral neurodynamic tests (NDT) are reliable for the assessment of neuropathies in somatic nerves, we aimed to validate a novel NDT to assess VN activity, namely, the VN-NTD.</p></sec><sec><title>Methods</title><p>In this cross-sectional double-blind, sex-balanced study, 30 participants (15 females) completed a checklist of autonomic dysfunction symptoms. During the VN-NDT administration, HR and symptoms (i.e., mechanical allodynia) were monitored in parallel to a real-time ultrasonography imaging (USI) and motion capture analysis of the neck. The VN-NDT impact on HR and its accuracy for autonomic symptoms reported in the last 7 days were tested.</p></sec><sec><title>Results</title><p>The VN-NDT induced a significant HR reduction of about 12 and 8 bpm in males and females [<italic>t</italic>(1, 119) = 2.425; <italic>p</italic> &lt; 0.017; η<sub>p</sub><sup>2</sup> = 0.047, 95% confidence interval (CI): 0.93–9.18], respectively. No adverse events were observed during VN-NDT. A substantial interexaminer agreement between the evaluators in symptoms induction by VN-NDT was detected [<italic>F</italic>(1, 119) = 0.540; <italic>p</italic> = 0.464; η<sub>p</sub><sup>2</sup> = 0.005, low effect]. Notably, mechanical allodynia accuracy for gastrointestinal dysfunctions was excellent (<italic>p</italic> &lt; 0.05; 95% CI: 0.52–0.73; <italic>p</italic> &lt; 0.001; 95% CI: 0.81–0.96).</p></sec><sec><title>Conclusions</title><p>The novel VN-NDT is a valid and accurate test capable of detecting VN activation with high sensitivity. Data provided are suitable for both sexes as a hallmark of HR variation due to VN normal response. The proposed VN-NDT may be reliable as daily routine neurological examination tests for the evaluation of neuropathic signs related to neuroinflammation of the VN.</p></sec><sec><title>Clinical Trial Registration</title><p><ext-link ext-link-type="uri" xlink:href="http://www.ClinicalTrials.gov" xmlns:xlink="http://www.w3.org/1999/xlink">www.ClinicalTrials.gov</ext-link>, identifier NCT04192877.</p></sec>