AUTHOR=Ingannato Assunta , Bagnoli Silvia , Mazzeo Salvatore , Bessi Valentina , Matà Sabrina , Del Mastio Monica , Lombardi Gemma , Ferrari Camilla , Sorbi Sandro , Nacmias Benedetta 

TITLE=Neurofilament Light Chain and Intermediate HTT Alleles as Combined Biomarkers in Italian ALS Patients

JOURNAL=Frontiers in Neuroscience

VOLUME=15

YEAR=2021

URL=https://www.frontiersin.org/journals/neuroscience/articles/10.3389/fnins.2021.695049

DOI=10.3389/fnins.2021.695049

ISSN=1662-453X

ABSTRACT=<sec><title>Objective</title><p>To study the possible implication of the two biomarkers, intermediate alleles (IAs) of the Huntingtin (HTT) gene and neurofilament light chain (NfL) levels in plasma, in amyotrophic lateral sclerosis (ALS) patients.</p></sec><sec><title>Methods</title><p>We analyzed IAs in a cohort of 106 Italian ALS patients and measured the plasma NfL levels in 20% of the patients of the cohort. We correlated the two biomarkers with clinical phenotypes.</p></sec><sec><title>Results</title><p>Intermediate alleles were present in 7.5% of the patients of our cohort, a frequency higher than that reported in general population. Plasma NfL levels increased with age at onset (<italic>p</italic> &lt; 0.05). Patients with bulbar onset (BO) had higher plasma NfL concentration (CI −0.61 to −0.06, <italic>p</italic> = 0.02) and a later age at onset of the disease (CI −24.78 to −4.93, <italic>p</italic> = 0.006) with respect to the spinal onset (SO) form. Additionally, two of the patients, with IAs and plasma NfL concentration lower with respect to normal alleles’ carriers, presented an age at onset higher than the mean of the entire cohort.</p></sec><sec><title>Conclusion</title><p>According to our findings, plasma NfL and IAs of HTT gene may represent potential biomarkers in ALS, providing evidence of a possible implication in clinical phenotype.</p></sec>