AUTHOR=Mourão Flávio Afonso Gonçalves , Guarnieri Leonardo de Oliveira , Amaral Júnior Paulo Aparecido , Carvalho Vinícius Rezende , Mendes Eduardo Mazoni Andrade Marçal , Moraes Márcio Flávio Dutra TITLE=A Fully Adapted Headstage With Custom Electrode Arrays Designed for Electrophysiological Experiments JOURNAL=Frontiers in Neuroscience VOLUME=15 YEAR=2022 URL=https://www.frontiersin.org/journals/neuroscience/articles/10.3389/fnins.2021.691788 DOI=10.3389/fnins.2021.691788 ISSN=1662-453X ABSTRACT=

Electrophysiological recordings lead amongst the techniques that aim to investigate the dynamics of neural activity sampled from large neural ensembles. However, the financial costs associated with the state-of-the-art technology used to manufacture probes and multi-channel recording systems make these experiments virtually inaccessible to small laboratories, especially if located in developing countries. Here, we describe a new method for implanting several tungsten electrode arrays, widely distributed over the brain. Moreover, we designed a headstage system, using the Intan® RHD2000 chipset, associated with a connector (replacing the expensive commercial Omnetics connector), that allows the usage of disposable and inexpensive cranial implants. Our results showed high-quality multichannel recording in freely moving animals (detecting local field, evoked responses and unit activities) and robust mechanical connections ensuring long-term continuous recordings. Our project represents an open source and inexpensive alternative to develop customized extracellular records from multiple brain regions.