AUTHOR=Ikeda Hiroyuki , Ishii Akira , Sano Kohei , Chihara Hideo , Arai Daisuke , Abekura Yu , Nishi Hidehisa , Ono Masahiro , Saji Hideo , Miyamoto Susumu 

TITLE=Activatable Fluorescence Imaging of Macrophages in Cerebral Aneurysms Using Iron Oxide Nanoparticles Conjugated With Indocyanine Green

JOURNAL=Frontiers in Neuroscience

VOLUME=14

YEAR=2020

URL=https://www.frontiersin.org/journals/neuroscience/articles/10.3389/fnins.2020.00370

DOI=10.3389/fnins.2020.00370

ISSN=1662-453X

ABSTRACT=<sec><title>Background and Purpose</title><p>Chronic inflammation is involved in the formation and enlargement of cerebral aneurysms (CAs), with macrophages playing a key role in the process. The present study evaluated visualization of macrophages present in CAs using an activatable fluorescent probe (IONP-ICG) comprising an iron oxide nanoparticles (IONPs) conjugated with indocyanine green (ICG).</p></sec><sec><title>Methods</title><p>IONP-ICG was intravenously administered to 15-week-old CA model rats (<italic>n</italic> = 8), and <italic>ex vivo</italic> near-infrared fluorescence (NIRF) imaging and histological assessment of exposed CAs and cerebral arteries were performed 48 h later. Similar evaluations were performed in the control group, which included CA model rats given IONPs or ICG (<italic>n</italic> = 8 each).</p></sec><sec><title>Results</title><p>ICG-derived NIRF signals were detected in three IONP-ICG group rats but not in IONP or ICG control groups. Among the three rats that exhibited signals, NIRF signal accumulation was observed in the CA of two rats and at the site of hemodynamic stress in the left posterior cerebral artery in one rat. Histologically, NIRF signals correlated strongly with macrophage localization. A total of 13 CAs formed in the IONP-ICG group. The number of macrophages in the CA wall was significantly greater in the two CAs that exhibited NIRF signals compared to the remaining 11 CAs that did not (<italic>P</italic> = 0.037). Moreover, all 11 CAs that did not exhibit NIRF signals were iron-negative, while the two CAs that exhibited NIRF signals were both iron-positive (<italic>P</italic> = 0.013).</p></sec><sec><title>Conclusion</title><p>NIRF imaging using an activatable IONP-ICG probe is feasible for detecting the macrophage-rich regions in CAs and the cerebral artery wall, which is considered an early lesion in the process of CA formation.</p></sec>