AUTHOR=Chen Lunhao , Cheng Daxiao , Chu Jiachen , Zhang Ting , Dong Zhuoer , Lou Huifang , Zhu Liya , Liu Yijun TITLE=A Novel Method to Image Macropinocytosis in Vivo JOURNAL=Frontiers in Neuroscience VOLUME=Volume 12 - 2018 YEAR=2018 URL=https://www.frontiersin.org/journals/neuroscience/articles/10.3389/fnins.2018.00324 DOI=10.3389/fnins.2018.00324 ISSN=1662-453X ABSTRACT=

Here we described an experimental protocol for in vivo imaging of macropinocytosis and subsequent intracellular events. By microinjection, we delivered fluorescence dextrans together with or without ATPĪ³S into transparent Drosophila melanogaster embryos. Using a confocal microscope for live imaging, we monitored the generation of dextran-positive macropinosomes and subsequent intracellular events. Our protocol provides a continent and reliable way for investigating macropinocytosis and its underlying mechanisms, especially when combined with genetic strategies.