AUTHOR=Saunders Norman R. , Dziegielewska Katarzyna M. , Møllgård Kjeld , Habgood Mark D. TITLE=Markers for blood-brain barrier integrity: how appropriate is Evans blue in the twenty-first century and what are the alternatives? JOURNAL=Frontiers in Neuroscience VOLUME=9 YEAR=2015 URL=https://www.frontiersin.org/journals/neuroscience/articles/10.3389/fnins.2015.00385 DOI=10.3389/fnins.2015.00385 ISSN=1662-453X ABSTRACT=
In recent years there has been a resurgence of interest in brain barriers and various roles their intrinsic mechanisms may play in neurological disorders. Such studies require suitable models and markers to demonstrate integrity and functional changes at the interfaces between blood, brain, and cerebrospinal fluid. Studies of brain barrier mechanisms and measurements of plasma volume using dyes have a long-standing history, dating back to the late nineteenth-century. Their use in blood-brain barrier studies continues in spite of their known serious limitations in