Rupert W. Overall
Robert W. Williams
J. Alexander Heimel- 1Center for Regenerative Therapies Dresden, Genomics of Regeneration, Technische Universität Dresden, Dresden, Germany
- 2Department of Genetics, Genomics and Informatics, University of Tennessee Health Science Center, Memphis, TN, USA
- 3Cortical Structure and Function Group, Netherlands Institute for Neuroscience, Amsterdam, Netherlands
Modern biology is marked by the availability of enormous quantities of raw data. Advances in computer capacity and internet bandwidth mean that much of this information is now stored online, and most such data repositories are also open access. Together, these factors have opened up a goldmine of data to anyone with an internet connection. One of the greatest strengths of the available resources is their diversity; freely-accessible repositories exist, for example, for gene sequence, mRNA expression, protein binding, imaging, and text mining results. Standardized annotation schemes have also improved compatibility across different fields. The ability to obtain a wealth of information for any gene and its molecular interactions now enables a whole new realm of analysis beyond that originally envisaged by the individual contributors.
In fact, so much untapped data exists that primary research can now conceivably be carried out purely in silico. Because this approach to science utilizes data already available in the public domain and requires only a laptop connected to the internet, analyses are not subject to the constraints of a traditional “bricks-and-mortar” laboratory. Collaborating scientists may be located anywhere in the world, they have no need to invest in further data generation, and may be largely independent of their local funding environment. These factors thus open up access to the science to a wider research community than ever before.
Despite the wealth of public data, often accessible through user-friendly portals, there is still a “missing link” between data and discovery. Few researchers are aware of the resources or how to use them effectively. Thus, the bottleneck has shifted from the generation of raw data to the development of effective ways to explore, mine, and integrate existing information.
In September 2013, a 1-week workshop was held to introduce researchers and students to the use of such online resources (visit https://sites.google.com/site/neuroinformaticsjamboree/ for details and a list of resource links). Participants were shown how key resources can be exploited as either supporting data for existing projects or for hypothesis generation to kick-start new directions in their research (see the associated Workshop Report, Heimel et al., 2014). As an extension of this workshop, an experiment in collaborative science was undertaken, in which researchers were invited to investigate workflows with which online resources could be mined to yield new hypotheses.
The manuscripts resulting from this experiment, and presented in this Research Topic, show that online data resources in the hands of energetic investigators can yield interesting and relevant results in very short order. Drafts of these papers were all produced from scratch in 1 week and following significant work over the following year, all groups successfully converted their drafts into peer-reviewed publications. The six resulting manuscripts present a varied range of insights into the genetics of brain function and disease.
Vied and colleagues have undertaken a novel in silico investigation of neocortical development utilizing the Allen Institute's Developing Mouse Brain Atlas and the BrainSpan Atlas of the Developing Human Brain. Based on correlating in situ expression profiles, they propose a number of candidate genes with expected roles in embryonic development of the neocortex (Vied et al., 2014). Lotan and colleagues have integrated catalogs of genes associated with psychiatric disorders to search for common processes. They show that many genes associated with multiple disorders are localized to the postsynaptic density (Lotan et al., 2014). Pietrzykowski and Spijker investigated another aspect of neuropsychiatric disorders, impulsivity, by searching the GeneNetwork database for behavior and expression correlates to identify several microRNAs with putative roles in the regulation of impulse control (Pietrzykowski and Spijker, 2014). Capurro and colleagues used a novel approach to investigate genes involved in movement disorders. They employed computational deconvolution of gene expression data from the brains of Huntington and Parkinson patients to identify genes and pathways with altered expression in these disorders (Capurro et al., 2014). To explore gene regulation in autism, van de Lagemaat and colleagues studied transcriptional changes in synaptic transmission pathways. Using gene lists curated from the literature and published databases, they discovered an age-dependent imbalance in expression of genes associated with excitatory and inhibitory synapses (van de Lagemaat et al., 2014). Ashbrook and colleagues searched for novel genes associated with adult hippocampal neurogenesis. Defining a list of genes correlating with nestin, an established precursor cell marker, and highly expressed in the hippocampal dentate gyrus, they identified potential novel genes associated with the proliferating precursor cell population (Ashbrook et al., 2014).
The wide sample of web-based data sets and tools used in these studies is certainly not an exhaustive list, and many more resources exist—most of which are freely accessible. While the body of public data is dominated by large-scale initiatives, even more data are potentially available as part of smaller projects—although accessibility is still dependent on improving data-sharing standards (Ferguson et al., 2014; Sejnowski et al., 2014). We feel it is important that the studies presented in this Research Topic relied solely on existing public data. This fact demonstrates that the utility of such data sets does not need to end with initial publication—as has traditionally been the case in the past.
With six solid contributions to the literature, we feel that the experiment has been a success. We hope that this project will inspire other researchers to take advantage of the online resources available to them to inspire and complement their experimental work.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
References
Ashbrook, D. G., Delprato, A., and Grellmann, C. (2014). Transcript co-variance with Nestin in two mouse genetic reference populations identifies Lef1 as a novel candidate regulator of neural precursor cell proliferation in the adult hippocampus. Front. Neurosci. 8:418. doi: 10.3389/fnins.2014.00418
Capurro, A., Bodea, L., Schaefer, P., Luthi-Carter, R., and Perreau, V. M. (2014). Computational deconvolution of genome wide expression data from Parkinson's and Huntington's disease brain tissues using population-specific expression analysis. Front. Neurosci. 8:441. doi: 10.3389/fnins.2014.00441
PubMed Abstract | Full Text | CrossRef Full Text | Google Scholar
Ferguson, A. R., Nielson, J. L., Cragin, M. H., Bandrowski, A. E., and Martone, M. E. (2014). Big data from small data: data-sharing in the “long tail” of neuroscience. Nat. Neurosci. 17, 1442–1447. doi: 10.1038/nn.3838
PubMed Abstract | Full Text | CrossRef Full Text | Google Scholar
Heimel, J. A., Overall, R. W., and Williams, R. W. (2014). Workshop Report: INCF short course on neuroinformatics, neurogenomics and brain disease, 14–21 september 2013. Front. Neurosci. 8:449. doi: 10.3389/fnins.2014.00449
Lotan, A., Fenckova, M., Bralten, J., Alttoa, A., Dixson, L., Williams, R. W., et al. (2014). Neuroinformatic analyses of common and distinct genetic components associated with major neuropsychiatric disorders. Front. Neurosci. 8:331. doi: 10.3389/fnins.2014.00331
Pietrzykowski, A., and Spijker, S. (2014). Impulsivity and comorbid traits: a multi-step approach for finding putative responsible microRNAs in the amygdala. Front. Neurosci. 8:389. doi: 10.3389/fnins.2014.00389
PubMed Abstract | Full Text | CrossRef Full Text | Google Scholar
Sejnowski, T. J., Churchland, P. S., and Movshon, J. A. (2014). Putting big data to good use in neuroscience. Nat. Neurosci. 17, 1440–1441. doi: 10.1038/nn.3839
PubMed Abstract | Full Text | CrossRef Full Text | Google Scholar
van de Lagemaat, L. N., Nijhof, B., Bosch, D. M., Kohansal-Nodehi, M., Keerthikumar, S., and Heimel, J. A. (2014). Age-related decreased inhibitory vs. excitatory gene expression in the adult autistic brain. Front. Neurosci. 8:394. doi: 10.3389/fnins.2014.00394
PubMed Abstract | Full Text | CrossRef Full Text | Google Scholar
Keywords: data analysis, online resources, transcriptomics, proteomics, brain disease, systems genetics
Citation: Overall RW, Williams RW and Heimel JA (2015) Collaborative mining of public data resources in neuroinformatics. Front. Neurosci. 9:90. doi: 10.3389/fnins.2015.00090
Received: 20 December 2014; Accepted: 03 March 2015;
Published: 18 March 2015.
Edited by:
Ruth Luthi-Carter, University of Leicester, UKReviewed by:
Elizabeth A. Thomas, The Scripps Research Institute, USACopyright © 2015 Overall, Williams and Heimel. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Rupert W. Overall, rupert.overall@crt-dresden.de