Quantitative SISCOM Assessment for Epileptogenic Zone Localisation: Insights from a Multicentre Study Comparing Two Software Platforms in Temporal Lobe Epilepsy

Carla Oliveira Young ^1* Brunno Machado De Campos ¹ Edna Marina de Souza ¹ Sergio Querino Brunetto ¹ Maria Julia de Oliveira Santos Gualberto ² Leonardo Alexandre-Santos ² Marcos Geraldo Merichelo de Oliveira ² Marina Koutsodontis Machado Alvim ¹ Fernando Cendes ¹ Elba Etchebehere ¹ Lauro Wichert-Ana ² Bárbara Juarez Amorim ^1*

• ¹ State University of Campinas, Campinas, Brazil
• ² University of São Paulo, Ribeirão Preto, Ribeirão Preto, São Paulo, Brazil

Pharmacoresistant epilepsy affects around one-third of individuals with epilepsy, requiring precise diagnosis, particularly in cases where surgical resection of the epileptogenic zone (EZ) is an option. Functional imaging techniques, such as ictal-interictal subtraction SPECT coregistered to MRI (SISCOM), have proven useful in pre-surgical evaluation by improving EZ localisation accuracy. However, the widespread use of SISCOM is limited by the high costs and technical complexity of commercial software. Statistical Parametric Mapping (SPM) has been demonstrated to be a viable alternative for SISCOM analysis, displaying the potential for costeffective EZ localisation. Materials and methods: In this retrospective study, we evaluated patients with pharmacoresistant temporal lobe epilepsy from two reference centres of epilepsy in Brazil, who underwent ictal and interictal SPECT imaging as part of their pre-surgical evaluation, achieving favourable outcomes (Engel I or II) after surgical resection. The EZ reference standard was determined according to anatomopathological findings and good clinical outcomes. SISCOM was performed using a semi-automated approach with Statistical Parametric Mapping (SPM) and a proprietary software -Analyze. Data from each method were compared to the EZ reference standard and classified as concordant, partially concordant, or discordant. Results: We included 20 patients, 14 (70%) with left temporal lobe epilepsy and six (30%) with right temporal lobe epilepsy.Hippocampal sclerosis was the most common pathology (80%). Both SPM and Analyze were concordant with the EZ reference standard in 14 cases (70%), showing no difference in sensitivity between the methods. However, SPM generated smaller, more localised clusters, while Analyze produced larger clusters with broader spatial coverage. Concordance between the two methods was poor (Kappa = 0.0179), reflecting methodological differences. Conclusion: This study evidences technical differences between SISCOM performed with SPM and Analyze, but with similar sensitivity (70%) for EZ localisation. Further studies with larger sample sizes are required to confirm these findings. The data presented here suggest that SISCOM-SPM, due to its rapid and semi-automated workflow, may offer a practical and accessible alternative to proprietary software for epilepsy surgical planning.