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ORIGINAL RESEARCH article
Front. Neurol.
Sec. Stroke
Volume 16 - 2025 | doi: 10.3389/fneur.2025.1533558
Unveiling BID: A Key Biomarker in Apoptosis Post-Intracerebral Hemorrhage
Provisionally accepted
Kun Dai 1
Hong-Rong Zhang 1
Shuai-Yu Ren 1
Ming-Pei Zhao 2 Neng Wang 2
Hong-Zhi Gao 3 De-Zhi Kang 2
Zong-Qing Zheng 2*- 1 The First Affiliated Hospital of Soochow University, Suzhou, China
- 2 First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian Province, China
- 3 The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian Province, China
Background: Apoptosis plays a significant role in secondary brain injury following intracerebral hemorrhage (ICH). Currently, the mechanisms related to cell apoptosis after cerebral hemorrhage are still under investigation. Methods: We identified differentially expressed genes (DEGs) between human ICH patients and normal individuals from the GEO database and conducted GO and KEGG functional enrichment analyses on these DEGs. We then constructed a PPI network and used the MECDE algorithm to identify key genes potentially involved in apoptosis after ICH. Additionally, we identified miRNAs that might regulate apoptotic genes in an mRNA-miRNA interaction network.Finally, we validated the bioinformatics results in a rat ICH model. Results: In the human ICH model, 645 DEGs were identified. GO and KEGG analyses indicated that these DEGs are primarily involved in immune response, inflammatory response, and apoptosis. GSEA analysis showed significant enrichment of DEGs in the apoptotic process. By comparing with apoptosis-related genes in the MSigDB database, we identified 110 apoptosis-related genes among the 645 DEGs. Further PPI and MOCDE analyses of these apoptosis-related genes revealed that BID might be a key gene involved in apoptosis after ICH, which was validated within the rat model of ICH. The mRNA-miRNA interactions network construction suggested that miR1225-3p may be an important miRNA involved in regulating BID expression after ICH. Conclusion: BID plays a critical role in the regulation of apoptosis following intracerebral hemorrhage and serves as a key biomarker in the apoptotic process after
Keywords: ICH, Apoptosis, Bioinformatics analysis, microRNA, BID
Received: 24 Nov 2024; Accepted: 17 Feb 2025.
Copyright: © 2025 Dai, Zhang, Ren, Zhao, Wang, Gao, Kang and Zheng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Zong-Qing Zheng, First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian Province, China
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