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ORIGINAL RESEARCH article

Front. Neurol.
Sec. Stroke
Volume 15 - 2024 | doi: 10.3389/fneur.2024.1512720
This article is part of the Research Topic Blood-Based Cellular and Molecular Biomarkers in Acute Ischemic Stroke and Hemorrhagic Stroke - Volume II View all 10 articles

Characteristics of Peripheral Immune Response induced by Large-Vessel Occlusion in Patients with Acute Ischemic Stroke

Provisionally accepted
Ling Ma Ling Ma 1Bin Sun Bin Sun 2Chenliu Fan Chenliu Fan 3Juan Xiao Juan Xiao 3Maomao Geng Maomao Geng 2Jie Liu Jie Liu 4Jiang Runze Jiang Runze 5Yang Jiang Yang Jiang 3*Dianwei Liu Dianwei Liu 2*
  • 1 Department of Clinical Laboratory, Second Hospital of Shandong University, Jinan, Shandong Province, China
  • 2 Central Hospital Affiliated to Shandong First Medical University, Jinan, China
  • 3 Hematology Department, The Second Hospital of Shandong University, Jinan, China
  • 4 Department of Clinical Laboratory, WeiHai Haida Hospital, Weihai, China
  • 5 Jinan Biomedical Industry Academy of Shandong First Medical University, Jinan, China

The final, formatted version of the article will be published soon.

    Despite improvements in the treatment of acute ischemic stroke (AIS), some patients still suffer from functional impairments, indicating the poor understanding of pathophysiologic process of AIS. Inflammation plays an important role in the pathophysiology of AIS. The purpose of the study was to investigate the peripheral inflammation in different subtypes of AIS. Here, retrospective data from AIS with large vessel occlusion (LVO) and small vessel occlusion (SVO), and healthy controls, were initially analyzed. Then, flow cytometry was performed to evaluate the levels of peripheral naïve and memory T-cells. Finally, we characterized the T cell receptors (TCR) repertoire using high-throughput sequencing. Elevated levels of leukocytes, neutrophils, and neutrophil-to-lymphocyte ratio (NLR), and decreased levels of lymphocytes were found in LVO group than that in SVO group, which were correlated with the severity of LVO. In addition, higher percentages of both effector memory (Tem) and central memory (Tcm) T cells, and lower percentage of naïve T cells in CD4+ and CD8+ T cells, were found in LVO group than that in SVO and healthy groups. Moreover, impaired TCR diversity, and different abundances of V-J gene combinations and amino acid sequences, were found in LVO as compared with healthy group, which would be potential biomarkers for LVO diagnosis. In conclusion, AIS with LVO can rapidly induce peripheral immune response, which provides new insight into the understanding of pathophysiology of AIS.

    Keywords: Acute ischemic stroke, Large vessel occlusion, T cell receptors repertoire, CD45, T cell

    Received: 17 Oct 2024; Accepted: 27 Nov 2024.

    Copyright: © 2024 Ma, Sun, Fan, Xiao, Geng, Liu, Runze, Jiang and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Yang Jiang, Hematology Department, The Second Hospital of Shandong University, Jinan, China
    Dianwei Liu, Central Hospital Affiliated to Shandong First Medical University, Jinan, China

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