AUTHOR=Weng Yin-Hua , Yu Wen-Tao , Luo Yan-Ping , Liu Chao , Gu Xi-Xi , Chen Huo-Ying , Liu Hong-Bo 

TITLE=Association between miR-365 polymorphism and ischemic stroke in a Chinese population

JOURNAL=Frontiers in Neurology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/neurology/articles/10.3389/fneur.2023.1260230

DOI=10.3389/fneur.2023.1260230

ISSN=1664-2295

ABSTRACT=<sec><title>Background</title><p>Ischemic stroke (IS) represents a major cause of morbidity and mortality across the globe. The aberrant expression of miR-365 has been found to be implicated in a wide array of human diseases, including atherosclerosis and cancer. Studies on single-nucleotide polymorphisms (SNPs) in miRNA genes can help gain insight into the susceptibility to the condition. This study aimed to examine the relationship between miR-365 SNPs and the risk of IS.</p></sec><sec><title>Methods</title><p>The study recruited 215 IS patients and 220 controls. The SNPscans genotyping was employed to genotype three polymorphic loci (rs121224, rs30230, and rs178553) of miR-365. The relative expression of miR-365 in peripheral blood mononuclear cells of the patients and controls was determined by using real-time quantitative PCR.</p></sec><sec><title>Results</title><p>The miR-365 rs30230 polymorphism exhibited a significant association with the risk of developing IS (TC vs. CC: adjusted OR = 0.55, 95% CI: 0.33-0.92, <italic>P</italic> = 0.022; TT vs. CC: adjusted OR = 0.34, 95% CI: 0.14–0.85, <italic>P</italic> = 0.021; TC +TT vs. CC: adjusted OR = 0.51, 95% CI: 0.31–0.83, <italic>P</italic> = 0.007; T vs. C: adjusted OR = 0.57, 95% CI: 0.39–0.83, <italic>P</italic> = 0.004). Haplotype analysis revealed that the C-T-G haplotype was associated with a decreased risk of IS (OR = 0.68, 95% CI: 0.46–1.00, <italic>P</italic> = 0.047). Furthermore, miR-365 expression was significantly higher in IS patients than in controls (<italic>P</italic> &lt; 0.001). Interestingly, patients with rs30230 TC or TT genotypes had lower miR-365 levels compared to their counterparts with CC genotypes (<italic>P</italic> &lt; 0.001).</p></sec><sec><title>Conclusions</title><p>The miR-365 rs30230 polymorphism might bear an association with IS susceptibility in the Chinese population, and the rs30230 TC/TT genotype might be a protective factor against IS.</p></sec>