Lin Yao
Sansong Chen
Zuan Yu
Tao Yu- Department of Neurosurgery, The Translational Research Institute for Neurological Disorders, The First Affiliated Hospital of Wannan Medical College (Yijishan Hospital), Wuhu, China
Multifocal brain abscesses caused by invasive Streptococcus intermedia are relatively rare. Here, we present a 67-year-old male was admitted to the hospital for unconsciousness and fever. The computed tomography (CT) examination showed multiple intracranial space-occupying and “cavity-like” changes in the right lower lung. The examination of cerebrospinal fluid (CSF) was consistent with typical bacterial meningitis, CSF analyses revealed leukocytosis (10,300 × 106/L), elevated protein levels (140.39 mg/dL), decreased glucose levels (0.27 mmol/L), and normal chloride concentration level (120.2 mmol/L), however, pathogens were not detected in the cultures. Then, the CSF and sputum samples were analyzed using meta-genomic next-generation sequencing (mNGS), and S. intermedia was detected in both samples. We adjusted the use of antibiotics according to the results of mNGS in time. After anti-infective treatment, the patient achieved good treatment results in a very short time. This case highlights the mNGS can identify pathogens of brain abscess, and provide strong evidence for clinical diagnosis and treatment strategy.
Introduction
Brain abscess is often a localized brain infection caused by the spread of nearby infections, such as otitis, mastoiditis, sinusitis, neurosurgery or traumatic brain injury, and blood spread (1). It has a prevalence of ~0.9 per 10,000 people per year, with a 1-year mortality rate of ~20%. Common clinical symptoms of brain abscess include headache, fever, vomiting, cramps, and focal neurological deficits (2).
S. intermedia exists mainly exists in the human oral cavity, throat, and gastrointestinal tract. S. intermedius can cause gingival abscess and aspiration pneumonia, leading to sepsis, endocarditis, and lung abscesses. S. intermedia has been reported to cause brain abscesses and often coexist with anaerobic bacteria (3, 4). Herein, we present a case of multifocal brain abscess that showed the diagnosis and treatment of the patient.
Case presentation
A 67-year-old male was admitted to our hospital due to unconsciousness and fever. The patient had fever symptoms 3 days before admission, the highest temperature was 39.6°C, and no special treatment was given. The patient had a history of drunkenness and aspiration 1 week before admission. One day before admission, the patient suddenly lost consciousness with quadriplegia. Physical examination: the patient was in a deep coma; the diameter of the bilateral pupils was 2 mm; the Glasgow score was E1V1M3; and the muscle strength of the limbs was grade 2. Blood analyses revealed evidence of leukocytosis (33.6 × 109/L) and elevated C-reactive protein (CRP) levels (199.80 mg/L), neutrophils percentage (93.80%), and procalcitonin range (1.58 ng/ml). Head computed tomography (CT) showed multiple intracranial space-occupying edema, and chest CT showed a right upper lobe space occupying the cavity. Brain magnetic resonance imaging (MRI) showed multiple divergent intracranial abscesses, which were low signal on the T1 weighted image and high signal on the T2 weighted image and were significantly enhanced. On the day of admission, the patient had difficulty breathing and was given mechanical ventilation with tracheal intubation (Figure 1). On day 3, a tracheotomy was performed. The patient had no history of tuberculosis, sputum tuberculosis and tuberculosis infection T cell detection was negative.
Figure 1. Head CT on admission shows multiple annular nodules in the left thalamus, and the edema is obvious (A). The chest CT shows a space-occupying lesion in the cavity in the upper lobe of the right lung (B). The head MRI on May 12 showed that scattered multiple signals can be seen in the bilateral cerebral hemispheres, thalamus, and brainstem, which were low signal on the T1 weighted image and high signal on the T2 weighted image and diffusion-weighted imaging (C–E). Magnetic Resonance Spectroscopy (left thalamus) showed that the Cho peak, Cr peak, and NAA peak decreased, the Cho/Cr ratio was 0.709, the Cho/NAA ratio was 0.847, and the LL peak increased significantly (F). Brain abscess was significantly enhanced (G–I).
To determine the patient's intracranial infection, we performed a lumbar puncture on the third day after admission. Cerebrospinal fluid (CSF) analyses revealed leukocytosis (10,300 × 106/L), elevated protein levels (140.39 mg/dL), decreased glucose levels (0.27 mmol/L), and normal chloride concentration level (120.2 mmol/L). However, pathogens were not detected in the cultures, and Gram staining assays were prepared from CSF, sputum, and blood samples. We used vancomycin (2 g every 12 h, ivgtt) combined with meropenem (1 g every 8 h, ivgtt) and ornidazole (0.5 g every 12 h, ivgtt) as an empirical anti-infection treatment. We used mNGS to identify pathogens in CSF and alveolar lavage fluid on 5th day after admission (Table 1). We stopped vancomycin and meropenem, then switched to ceftriaxone (2 g every 24 h, ivgtt) combined with amikacin (0.4 g every 12 h, ivgtt) for anti-infection treatment.
Table 1. List of macro genomic pathogens detected in alveolar lavage fluid and cerebrospinal fluid (CSF) samples on 5 days after admission.
The patient's consciousness and limb muscle strength gradually improved during the subsequent treatment. Two weeks after admission, the patient's body temperature was normal, and no more than 39°C. Celsius Routine blood leukocyte count, neutrophil ratio, and procalcitonin levels decreased gradually to normal levels. We performed lumbar puncture during hospitalization at the third week after admission, which showed that the patient's intracranial pressure was 180 mmH2O and the color of the CSF was light blood and clear; CSF analyses showed normal leukocyte count (15 × 106/L), elevated protein levels (72.3 mg/dL), and normal glucose levels (4.37 mmol/L) and chloride concentration level (119 mmol/L). We then sequenced the CSF again at 4 weeks after admission, suggesting that S. intermedia could still be detected, but its relative abundance decreased from 39.34 to 5.62% (Table 2). We continued to use ceftriaxone in the treatment of intracranial infection until the patient's body temperature was normal for 2 weeks, we stopped all antibiotics at 6 weeks after admission (Table 3).
Table 2. List of macro genomic pathogens detected in cerebrospinal fluid (CSF) samples at 4 weeks after admission.
Table 3. Timeline.
After 8 weeks, the patient's consciousness became clear, and the muscle strength of the limbs recovered to grade 4. CT showed that the size of the lesion and the degree of edema improved significantly, the patient's upper right pneumonia was better than before, and the patient was discharged at the 10 weeks after admission (Table 3). The 3-month follow-up head MRI showed that the brain abscess had basically disappeared (Figure 2).
Figure 2. CT after 2 months of treatment. The size of the lesion and the degree of edema improved significantly (A). The patient's upper right pneumonia is better than before (B). The patient MRI in August 2021 showed that the abscess was significantly absorbed and improved (C–F).
Discussion
Multiple intracranial lesions accompanied by cavitation changes in the lungs need to rule out lung cancer brain metastases and tuberculous brain abscesses. The main symptom of the patient was high fever. Combined with the patient's laboratory examination, the first consideration was inflammatory disease. Tuberculous brain abscesses and bacterial brain abscesses have similar clinical and conventional MRI findings, both manifest as ring-enhancing cystic space-occupying lesions, which require further differential diagnosis with the help of clinical manifestations, biopsy, pathology, etc (5). Combined with the patient's medical history, laboratory examination, and imaging examination, we considered that the patient had a bacterial brain abscess.
Regarding the choice of the treatment plan for brain abscess, it is necessary to comprehensively evaluate the patient's clinical status and abscess. In this case, most of the intracranial lesions were divergent, and most of the abscesses were <1 cm in diameter, most of which were in the functional area of the brain, and a stereotactic puncture and craniotomy could not be performed. Therefore, it is important to choose a targeted drug treatment.
According to the overall etiological characteristics of intracranial infection in recent years, the distribution is slightly different in different regions and years. The etiology of brain abscesses is still dominated by positive bacteria, and the most common pathogens are Streptococcus and Staphylococcus. In a retrospective study, 332 patients with brain abscess with positive cultures were counted, and it was found that the most common bacteria were Streptococcus, Staphylococcus, and Proteus (6).
The positive rate of bacteriological culture in patients with brain abscesses was low. In a 10-year retrospective study, the study found that only 34 cases were positive in pus culture, accounting for 25.76% of the total number of confirmed patients (7). Empirical antimicrobial therapy recommended vancomycin combined with cephalosporins or carbapenems against Pseudomonas. However, it should be noted that long-term use of vancomycin and meropenem may cause complications such as neutropenia and imbalance in the bacterial population. The current view is that mNGS has certain advantages over traditional methods in central nervous system infection, improves the positive rate, and shortens the time of diagnosis (8, 9). In this case, Burkholderia cepacia, S. intermedia, and Pseudomonas aeruginosa were detected in alveolar lavage fluid samples from patient with mNGS, and S. intermedia was detected in patient CSF samples. The relative abundance of Burkholderia cepacia in bronchoalveolar lavage was higher than in S. intermedia. However, S. intermedia was detected in the CSF and alveolar lavage fluid, so we determined that S. intermedia may be the pathogen, and Burkholderia cepacia may be the hospital-acquired infectious bacteria.
Bacteria can invade the brain through direct dissemination or hematogenous dissemination, direct dissemination accounts for 20–60% of intracranial infections, bacteremic dissemination usually causes multiple lesions (1, 10). S. intermedia is part of the normal microbiota and are found at various mucosal sites in the respiratory (11). Brain abscesses are frequently caused by oral cavity bacteria (12). The patient had a history of drunkenness and aspiration 1 week before admission. We speculate that the patient's pneumonia due to aspiration, and the S. intermedia entered the patient's brain through blood dissemination, resulting in multiple brain abscesses in the patient.
A review of brain abscesses caused by invasive Streptococcus intermedia pointed out that most commonly prescribed antibiotic regimens were a combination of ceftriaxone and metronidazole alone (11). Therefore, we stopped vancomycin and meropenem, and switched to ceftriaxone combined with amikacin (Anti-pulmonary infection) for anti-infection treatment.
According to meta-analysis and retrospective study, more central nervous system infections can be correctly treated by mNGS (13–15). Currently, the application of mNGS in central nervous system infections is mainly based on case reports, and there are few large-scale studies to be referred to (16–18).
In summary, we report a patient with multifocal brain abscesses caused by S. intermedia; the patient had multiple failed cultures of pathogens from the patient's blood and CSF samples, and the mNGS analysis approach was used, we adjusted the use of antibiotics in time, and finally, the patient achieved good treatment results in a very short time.
Conclusions
mNGS has certain advantages in identifying brain abscesses; it can effectively avoid delaying the diagnosis and treatment of patients due to the lack of pathogens in routine culture. We believe that mNGS will provide greater help to neurosurgeons in their future work.
Data availability statement
The original contributions presented in the study are included in the article/supplementary material, further inquiries can be directed to the corresponding author/s.
Ethics statement
The studies involving human participants were reviewed and approved by the Ethics Committee of the First Affiliated Hospital of Wannan Medical College (Yijishan Hospital). The patients/participants provided their written informed consent to participate in this study. Written informed consent was obtained from the patient's legal representative for the publication of any potentially identifiable images or data included in this article.
Author contributions
SC and ZY participated in the collection of data and drafted the manuscript. LY collected the data for case presentation. TY reviewed the literature and participated in its design. All authors read and approved the final version of the manuscript.
Funding
This work was supported by the Key Research and Development Program of Anhui Province (201904a07020034) and the Peak Training Program for Scientific Research of Yijishan Hospital, Wannan Medical College (GF2019G05).
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher's note
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References
1. Brouwer MC, Tunkel AR, McKhann GM, van de Beek D. Brain abscess. N Engl J Med. (2014) 371:447–56. doi: 10.1056/NEJMra1301635
2. Hsieh TC, Lin CC, Wu LS. Long-term use of valproic acid reduced mortality in bipolar disorder patients in a Taiwanese population: an association analysis using the national health insurance research database (NHIRD). J Psychiatr Res. (2021) 149:339–43. doi: 10.1016/j.jpsychires.2021.11.021
3. Fransson M, Helldén A, Östholm Balkhed Å, Nezirević Dernroth D, Ha M, Haglund M, et al. Case report: Subtherapeutic vancomycin and meropenem concentrations due to augmented renal clearance in a patient with intracranial infection caused by Streptococcus intermedius. Front Pharmacol. (2021) 12:728075. doi: 10.3389/fphar.2021.728075
4. Sinha D, Sun X, Khare M, Drancourt M, Raoult D, Fournier PE. Pangenome analysis and virulence profiling of Streptococcus intermedius. BMC Genomics. (2021) 22:522. doi: 10.1186/s12864-021-07829-2
5. Singh SK, Hasbun R. Neuroradiology of infectious diseases. Curr Opin Infect Dis. (2021) 34:228–37. doi: 10.1097/QCO.0000000000000725
6. Campioli CC, Almeida NE, O'Horo JC, Wilson WR, Cano E, DeSimone DC, et al. Diagnosis, management, and outcomes of brain abscess due to gram-negative versus gram-positive bacteria. Int J Infect Dis. (2022) 115:189–94. doi: 10.1016/j.ijid.2021.12.322
7. Wu S, Wei Y, Yu X, Peng Y, He P, Xu H, et al. Retrospective analysis of brain abscess in 183 patients: a 10-year survey. Medicine (Baltimore). (2019) 98:e17670. doi: 10.1097/MD.0000000000017670
8. Stebner A, Ensser A, Geißdörfer W, Bozhkov Y, Lang R. Molecular diagnosis of polymicrobial brain abscesses with 16S-rDNA-based next-generation sequencing. Clin Microbiol Infect. (2021) 27:76–82. doi: 10.1016/j.cmi.2020.03.028
9. Miller S, Chiu C. The role of metagenomics and next-generation sequencing in infectious disease diagnosis. Clin Chem. (2021) 68:115–24. doi: 10.1093/clinchem/hvab173
10. Thy M, Gaudemer A. d'Humières C, Sonneville R. Brain abscess in immunocompetent patients: recent findings. Curr Opin Infect Dis. (2022) 35:238–45. doi: 10.1097/QCO.0000000000000833
11. Issa E, Salloum T, Tokajian S. Streptococcus intermedius from normal flora to brain abscesses: a review. Front Microbiol. (2020) 11:826. doi: 10.3389/fmicb.2020.00826
12. Bodilsen J, Helweg Larsen J, Otto Jarløv J, Ziebell M, Ellermann-Eriksen S, Justesen US, et al. Dentist's visits and risk of brain abscess: A nationwide, population-based case control study. Clin Infect Dis. (2021) 2021:ciab1054. doi: 10.1093/cid/ciab1054
13. Wilson MR, Sample HA, Zorn KC, Arevalo S, Yu G, Neuhaus J, et al. Clinical metagenomic sequencing for diagnosis of meningitis and encephalitis. N Engl J Med. (2019) 380:2327–40. doi: 10.1056/NEJMoa1803396
14. Tao Y, Yan H, Liu Y. Diagnostic performance of metagenomic next-generation sequencing in pediatric patients: a retrospective study in a large children's medical center. Clin Chem. (2022) 68:1031–41. doi: 10.1093/clinchem/hvac067
15. Gu J, Chen L, Zeng C. A retrospective analysis of metagenomic next generation sequencing (mNGS) of cerebrospinal fluid from patients with suspected encephalitis or meningitis infections. J Healthc Eng. (2022) 21:5641609. doi: 10.1155/2022/5641609
16. Lu H, Du Y, Pan T. Gardnerella vaginalis purulent meningitis in an adolescent male: a case report. BMC Neurol. (2022) 22:217. doi: 10.1186/s12883-022-02733-y
17. Yang J, Xie S, Li J. Nocardia farcinica brain abscess caused by and diagnosed by metagenomic next-generation sequencing: a case report. Front Med. (2022) 9:803554. doi: 10.3389/fmed.2022.803554
Keywords: multiple brain abscesses, Streptococcus intermedia, meta-genomic next-generation sequencing, case report, cerebrospinal fluid
Citation: Yao L, Chen S, Yu Z and Yu T (2022) Multifocal brain abscesses caused by invasive Streptococcus intermedia: A case report. Front. Neurol. 13:893627. doi: 10.3389/fneur.2022.893627
Received: 01 April 2022; Accepted: 27 July 2022;
Published: 12 August 2022.
Edited by:
Peter R. Williamson, National Institutes of Health (NIH), United StatesReviewed by:
Han Xia, Hugobiotech Co. Ltd., ChinaKazuo Nakamichi, National Institute of Infectious Diseases (NIID), Japan
Copyright © 2022 Yao, Chen, Yu and Yu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Tao Yu, eXV0YW8mI3gwMDA0MDt5anN5eS5jb20=