AUTHOR=Liu Lin , Yi Xingyang , Luo Hua , Yu Ming 

TITLE=Inflammation and endothelial function relevant genetic polymorphisms in carotid stenosis in southwestern China

JOURNAL=Frontiers in Neurology

VOLUME=13

YEAR=2023

URL=https://www.frontiersin.org/journals/neurology/articles/10.3389/fneur.2022.1076898

DOI=10.3389/fneur.2022.1076898

ISSN=1664-2295

ABSTRACT=<sec><title>Aim</title><p>To evaluate the relationship between carotid stenosis with variants in genes referred to inflammation and endothelial function.</p></sec><sec><title>Methods</title><p>There was a multi-center, cross sectional survey in southwestern China. The eight communities were selected at random in southwestern China. The residents aged ≥40 years volunteered to participate in face-to-face survey. Subjects with at least three of the aforementioned eight stroke related risk factors or a history of stroke were classified as high-risk population for stroke. A total of 2,377 subjects were the high-risk population for stroke in the eight communities, and degree of carotid stenosis was assessed by carotid ultrasound. Genotypes of 6 variants in 3 genes related to inflammation and endothelial function were examined. Gene-gene interaction was analyzed by generalized multifactor dimensionality reduction (GMDR).</p></sec><sec><title>Results</title><p>Carotid stenosis were found in 295 (12.41%) subjects, of whom 51 (17.29%) had moderate or severe stenosis. According to multivariate logistic regression analysis, we found that HABP2rs7923349TT was independent risk factor for carotid stenosis (OR, 1.96, 95% CI: 1.22–3.13, <italic>P</italic> = 0.005) and ITGA2rs1991013AG and HABP2rs7923349TT were independent risk factors for moderate to severe carotid stenosis (OR, 2.28, 95% CI: 1.28–4.07, <italic>P</italic> = 0.005; OR, 2.90, 95% CI: 1.19–7.08, <italic>P</italic> = 0.019). GMDR analysis showed that there was a significant gene-gene interaction between <italic>ITGA2</italic> rs4865756 and <italic>HABP2</italic> rs7923349, and the high-risk interactive genotype in the two variants was independently associated with a higher risk for carotid stenosis after adjusting the covariates (OR,1. 42, 95% CI 1.10–1.84, <italic>P</italic> = 0.008).</p></sec><sec><title>Conclusions</title><p>Prevalence of carotid stenosis was very high in the high-risk stroke population in southwestern China. Variants in genes referred in endothelial function were associated with the carotid stenosis. The high—risk interactive genotype in <italic>ITGA2</italic> rs4865756 and <italic>HABP2</italic> rs7923349 was independently associated with a higher risk for carotid stenosis.</p></sec>