AUTHOR=Ferraldeschi Michela , Romano Silvia , Giglio Simona , Romano Carmela , Morena Emanuele , Mechelli Rosella , Annibali Viviana , Ubaldi Martina , Buscarinu Maria Chiara , Umeton Renato , Sani Gabriele , Vecchione Andrea , Salvetti Marco , Ristori Giovanni 

TITLE=Circulating hsa-miR-323b-3p in Huntington's Disease: A Pilot Study

JOURNAL=Frontiers in Neurology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/neurology/articles/10.3389/fneur.2021.657973

DOI=10.3389/fneur.2021.657973

ISSN=1664-2295

ABSTRACT=<p>The momentum of gene therapy in Huntington's disease (HD) deserves biomarkers from easily accessible fluid. We planned a study to verify whether plasma miRNome may provide useful peripheral “reporter(s)” for the management of HD patients. We performed an exploratory microarray study of whole non-coding RNA profiles in plasma from nine patients with HD and 13 matched controls [eight healthy subjects (HS) and five psychiatric patients (PP) to minimize possible iatrogenic impact on the profile of non-coding RNAs]. We found an HD-specific signature: downregulation of hsa-miR-98 (fold change, −1.5, <italic>p</italic> = 0.0338 HD vs. HS, and fold change, 1.5, <italic>p</italic> = 0.0045 HD vs. PP) and upregulation of hsa-miR-323b-3p (fold change, 1.5, <italic>p</italic> = 0.0007 HD vs. HS, and fold change, 1.5, <italic>p</italic> = 0.0111 HD vs. PP). To validate this result in an independent cohort, we quantify by digital droplet PCR (ddPCR) the presence of the two microRNA in the plasma of 33 HD patients and 49 matched controls (25 HS and 24 PP patients). We were able to confirm that hsa-miR-323b-3p was upregulated in HD and premanifest HD vs. HS and PP: the median values (first–third quartile) were 4.1 (0.9–10.53) and 5.8 (1.9–10.70) vs. 0.69 (0.3–2.75) and 1.4 (0.78–2.70), respectively, <italic>p</italic> &lt; 0.05. No significant difference was found for hsa-miR-98. To evaluate the biological plausibility of the hsa-miR-323b-3p as a component of the disease pathophysiology, we performed a bioinformatic analysis based on its targetome and the huntingtin (HTT) interactome. We found a statistically significant overconnectivity between the targetome of hsa-miR-323b-3p and the HTT interactome (<italic>p</italic> = 1.48e−08). Furthermore, there was a significant transcription regulation of the HTT interactome by the miR-323b-3p targetome (<italic>p</italic> = 0.02). The availability of handy, reproducible, and minimally invasive biomarkers coming from peripheral miRNome may be valuable to characterize the illness progression, to indicate new therapeutic targets, and to monitor the effect of disease-modifying treatments. Our data deserve further studies with larger sample size and longitudinal design.</p>