AUTHOR=Cao Zhentang , Wu Yufeng , Liu Genliang , Jiang Ying , Wang Xuemei , Wang Zhan , Feng Tao 

TITLE=Differential Diagnosis of Multiple System Atrophy-Parkinsonism and Parkinson's Disease Using α-Synuclein and External Anal Sphincter Electromyography

JOURNAL=Frontiers in Neurology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/neurology/articles/10.3389/fneur.2020.01043

DOI=10.3389/fneur.2020.01043

ISSN=1664-2295

ABSTRACT=<p><bold>Background and aim:</bold> Discriminating multiple system atrophy-parkinsonism (MSA-P) from Parkinson's disease (PD) is challenging. We aimed to provide a new method to make an identification between MSA-P and PD by combining biofluid marker with electrophysiology marker.</p><p><bold>Methods:</bold> The XYCQ EV Enrichment KIT was applied to extract extracellular vesicles (EVs) from saliva. The levels of α-syn which included total α-syn (α- syn<sub>Total</sub>), phosphorylated-ser129 α-syn (α-syn<sub>PS129</sub>) and oligomeric α-syn (α-syn<sub>Olig</sub>) in EVs of saliva were tested by new developed Electrochemiluminescence (ECL) assays. We collected multi-motor unit potential (MUP) of all participants who conducted external anal sphincter electromyography (EAS-EMG). The duration, phase, amplitude and satellite potential of EAS-EMG were analyzed. The Receiver operator characteristic (ROC) curve was adopted to analyze the diagnostic utility of α-syn in EVs of saliva, EAS-EMG for MSA-P.</p><p><bold>Results:</bold> In EVs of saliva, the α-syn<sub>Total</sub> concentrations were lower in MSA-P than PD (<italic>P</italic> = 0.003). No significant difference was shown in α-syn<sub>Olig</sub> and α-syn<sub>PS129</sub>. α-syn<sub>Total</sub> 4.46 pg/ng distinguished MSA-P from PD with area under the curve (AUC) 0.804. Compared with PD, the duration, phase and satellite potential of EAS-EMG in MSA-P were increased (<italic>P</italic> = 0.002, 0.008, 0.001). There was no significant difference in amplitude. ROC curve showed that the duration (AUC: 0.780), phase (AUC: 0.751), and satellite potential (AUC: 0.809) had both diagnostic value for MSA-P. The combination of α-syn<sub>Total</sub> in salivary EVs and EAS-EMG (including duration, phase and satellite potential) could efficiently make a differentiation between MSA-P and PD with sensitivity of 100% and specificity of 86%. The AUC value was 0.901.</p><p><bold>Conclusion:</bold> The study suggested the combination of α-syn<sub>Total</sub> in salivary EVs and EAS-EMG could help efficiently distinguish MSA-P from PD.</p>