AUTHOR=Xia Lei , Chen Mingjie , Bi Dan , Song Juan , Zhang Xiaoli , Wang Yangong , Zhu Dengna , Shang Qing , Xu Falin , Wang Xiaoyang , Xing Qinghe , Zhu Changlian 

TITLE=Combined Analysis of Interleukin-10 Gene Polymorphisms and Protein Expression in Children With Cerebral Palsy

JOURNAL=Frontiers in Neurology

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/neurology/articles/10.3389/fneur.2018.00182

DOI=10.3389/fneur.2018.00182

ISSN=1664-2295

ABSTRACT=<sec id="ST1"><title>Background</title><p>Interleukin-10 (IL-10) is an important anti-inflammatory and immunosuppressive cytokine, and it has indispensable functions in both the onset and development of inflammatory disorders. The association between persistent inflammation and the development of cerebral palsy (CP) has attracted much attention.</p></sec><sec id="ST2"><title>Objective</title><p>The purpose of this study was to investigate whether <italic>IL-10</italic> gene polymorphisms and plasma protein expression are associated with CP and to analyze the role of IL-10 in CP.</p></sec><sec id="ST3"><title>Methods</title><p>A total of 282 CP patients and 197 healthy controls were genotyped for <italic>IL-10</italic> polymorphisms (rs1554286, rs1518111, rs3024490, rs1800871, and rs1800896). Among them, 95 CP patients and 93 healthy controls were selected for plasma IL-10 measurement.</p></sec><sec id="ST4"><title>Results</title><p>The differences in the rs3024490 (<italic>p</italic> = 0.033) and rs1800871 (<italic>p</italic> = 0.033) allele frequencies of <italic>IL-10</italic> were determined between CP patients and controls. The frequencies of allele and genotype between CP patients with spastic tetraplegia and normal controls of <italic>IL-10</italic> polymorphisms showed significant differences for rs1554286, rs151811, rs3024490, rs1800871, and rs1800896 (<italic>p<sub>allele</sub></italic> = 0.015, 0.009, 0.006, 0.003, and 0.006, <italic>p<sub>genotype</sub></italic> = 0.039, 0.018, 0.027, 0.012, and 0.03, respectively). The plasma IL-10 protein level in CP patients was higher than normal controls (9.13 ± 0.77 vs. 6.73 ± 0.63 pg/ml, <italic>p</italic> = 0.017). IL-10 polymorphisms and protein association analysis showed that the TT genotype had higher plasma IL-10 protein levels compared to the GG + GT genotype at rs3024490 (11.14 ± 7.27 vs. 7.44 ± 6.95 pg/ml, <italic>p</italic> = 0.045, respectively) in CP cases.</p></sec><sec id="ST5"><title>Conclusion</title><p>These findings provide an important contribution toward explaining the pleiotropic role of IL-10 in the complex etiology of CP.</p></sec>