AUTHOR=Rennert Robert C. , Schäfer Richard , Bliss Tonya , Januszyk Michael , Sorkin Michael , Achrol Achal S. , Rodrigues Melanie , Maan Zeshaan N. , Kluba Torsten , Steinberg Gary K. , Gurtner Geoffrey C. 

TITLE=High-Resolution Microfluidic Single-Cell Transcriptional Profiling Reveals Clinically Relevant Subtypes among Human Stem Cell Populations Commonly Utilized in Cell-Based Therapies

JOURNAL=Frontiers in Neurology

VOLUME=7

YEAR=2016

URL=https://www.frontiersin.org/journals/neurology/articles/10.3389/fneur.2016.00041

DOI=10.3389/fneur.2016.00041

ISSN=1664-2295

ABSTRACT=<p>Stem cell therapies can promote neural repair and regeneration, yet controversy regarding optimal cell source and mechanism of action has slowed clinical translation, potentially due to undefined cellular heterogeneity. Single-cell resolution is needed to identify clinically relevant subpopulations with the highest therapeutic relevance. We combine single-cell microfluidic analysis with advanced computational modeling to study for the first time two common sources for cell-based therapies, human NSCs and MSCs. This methodology has the potential to logically inform cell source decisions for any clinical application.</p>