AUTHOR=Ranjan Amit , Mallick Birendra N.

TITLE=A Modified Method for Consistent and Reliable Golgi–Cox Staining in Significantly Reduced Time

JOURNAL=Frontiers in Neurology

VOLUME=1

YEAR=2010

URL=https://www.frontiersin.org/journals/neurology/articles/10.3389/fneur.2010.00157

DOI=10.3389/fneur.2010.00157

ISSN=1664-2295

ABSTRACT=<p>The two major limitations of Golgi–Cox method are that staining takes very long time and it is inconsistent. In this paper we describe a modification of the Golgi–Cox method, in which the tissue blocks were maintained at 37 ± 1°C during chromation for only 24 h and consistent staining of neurons in rat brain sections were observed. The method is simple, reproducible, rapid, inexpensive, and provides uniform staining with very good resolution of neuronal soma, dendrites as well as spines.</p>