Tracy Butler1*
Gloria C. Chiang1
Sumit Narayan Niogi1
Xiuyuan Hugh Wang1
Carly Skudin1
Emily Tanzi1Nimmi Wickramasuriya1Jonathan Spiegel1Thomas Maloney1Silky Pahlajani1
Liangdong Zhou1Simon Morim1
Henry Rusinek2Marc Normandin3
Jonathan P. Dyke1Edward K. Fung1
Yi Li1
Lidia Glodzik1
Qolamreza Ray Razlighi1
Sudhin A. Shah1
Mony de Leon1- 1Department of Radiology, Weill Cornell Medicine, New York, NY, United States
- 2New York University Grossman School of Medicine, New York, NY, United States
- 3Department of Radiology Harvard Medical School, Boston, MA, United States
Repeated mild Traumatic Brain Injury (TBI) is a risk factor for Chronic Traumatic Encephalopathy (CTE), characterized pathologically by neurofibrillary tau deposition in the depths of brain sulci and surrounding blood vessels. The mechanism by which TBI leads to CTE remains unknown but has been posited to relate to axonal shear injury leading to release and possibly deposition of tau at the time of injury. As part of an IRB-approved study designed to learn how processes occurring acutely after TBI may predict later proteinopathy and neurodegeneration, we performed tau PET using 18F-MK6240 and MRI within 14 days of complicated mild TBI in three subjects. PET radiotracer accumulation was apparent in regions of traumatic hemorrhage in all subjects, with prominent intraparenchymal PET signal in one young subject with a history of repeated sports-related concussions. These results are consistent with off-target tracer binding to blood products as well as possible on-target binding to chronically and/or acutely-deposited neurofibrillary tau. Both explanations are highly relevant to applying tau PET to understanding TBI and CTE. Additional study is needed to assess the potential utility of tau PET in understanding how processes occurring acutely after TBI, such as release and deposition of tau and blood from damaged axons and blood vessels, may relate to development CTE years later.
Introduction
Chronic Traumatic Encephalopathy (CTE), a neurodegenerative disorder associated with progressive neuropsychiatric decline in individuals who have suffered repeated mild traumatic brain injury (TBI), is currently diagnosable only at autopsy based on the presence of neurofibrillary tau tangles in the depths of brain sulci and surrounding blood vessels (McKee et al., 2014). Positron Emission Tomography (PET) now allows assessment of tauopathy in vivo. A small number of tau PET studies have revealed tau deposition months to years following TBI (repeated mild and single moderate-severe) and in subjects with clinically-probable CTE, (Stern et al., 2019; Ayubcha et al., 2021; Marklund et al., 2021) though differences between patients and controls are subtle, confounded by non-specific binding of early generation tracers, and not considered diagnostic at the individual level. The mechanism by which repeated TBI leads to CTE remains unknown but has been posited to relate to release of tau at the time of injury through axonal shearing. Tau levels in blood, CSF and interstitial fluid rise rapidly following TBI and can provide clinically relevant information about TBI severity and prognosis (Zetterberg, 2017). We designed a study to learn if tau PET performed shortly after TBI can improve understanding of how processes occurring acutely after TBI may predict later neurodegeneration. We report early results from this study, focusing on the first subject enrolled whose tau PET scan was markedly abnormal with prominent radiotracer uptake in regions of hemorrhage.
Methods
Participants and setting
As part of a study approved by the Weill Cornell Medicine IRB, adult subjects with complicated mild [GCS 13-15 with trauma-related CT abnormality such as intracranial hemorrhage or contusion (Marshall et al., 1991)] or moderate TBI were recruited from the Weill Cornell—New York Presbyterian Emergency Department to undergo PET and MRI neuroimaging within 14 days of injury. All subjects provided informed consent prior to participation.
Neuroimage acquisition
PET scans were acquired from 0 to 60 and 90 to 120 min on a Siemens Biograph PET/CT after rapid IV bolus injection of ~555 MBq 18F-MK6240.
MRI was acquired on a research-dedicated 3T GE system. Sequences included T1 BRAVO for PET coregistration (TR of 256*8.2 = 2,097 ms, TE = 3.2 ms. TI = 450 ms, Acquired Resolution = 1 x 1 x 1 mm interpolated to 0.5 x 0.5 x 0.5 mm; 180 sagittal slices) and 3D Spiral Arterial Spin Labeling (ASL; TR = 4,851 ms, TE = 10.6 ms, Acquired Resolution = 1.875 x 1.875 x 3.8 mm, 36 axial slices).
Neuroimage processing and analysis
After reconstruction and decay correction, PET images were motion corrected and coregistered with T1 MRI using validated methods (Tahmi et al., 2019). SUVr images for summed 90–120 min acquisition were generated with reference to cerebellar gray matter, defined by Freesurfer and eroded by 2 voxels to avoid partial volume effects.
Results
TBI subject characteristics are shown in Table 1. All subjects suffered complicated mild TBI with brief (<5 min) loss or alteration of consciousness with normal Glasgow Comas Scale (GCS) scores of 15 by the time of Emergency Department evaluation. As shown in Figure 1, all subjects' 18F-MK6240 PET scans were abnormal. Subject #1, with a history of repeated (10+) sports-related concussions, had two frontal areas of intense 18F-MK6240 uptake in the regions of MR-visible T1 hyperintense, hemorrhagic cerebral contusion and subdural hemorrhage. In this subject only, radiotracer accumulation appeared to extend into brain parenchyma. Subject #2 had probable extra-axial tracer accumulation along a parafalcine subdural hematoma adjacent to (normal off-target) tracer accumulation in skull. Subject #3 showed a small amount of extra-axial tracer accumulation in the region of epidural hematomas.
Table 1. Subject characteristics.
Figure 1. 18F-MK6240 PET and MRI performed within 14 days of complicated mild TBI in 3 subjects. Top panel shows T1 MRI and bottom panel shows 18F-MK6240 PET (SUVr) overlaid on MRI. (A) Subject #1: MRI shows hemorrhagic contusions in L frontal and L>R orbitofrontal regions. PET shows focal radiotracer accumulation within and surrounding these regions, with the L frontal lesion (white circle) showing a c-shaped configuration. (B) Subject #2: MRI shows parafalcine trace subdural hematoma, diffuse atrophy, and a developmental arachnoid cyst. PET signal in the region of subdural hematoma (white circle) may be present, but is difficult to differentiate from (normal, off-target) signal in adjacent dura. (C) Subject #3: MRI shows R anterior and L lateral temporal epidural hematomas. PET shows extra-axial radiotracer accumulation in these regions (L temporal = small white circle; R temporal = larger white circle).
Discussion
18F-MK6240 tau PET performed shortly after complicated mild TBI in three subjects revealed focal radiotracer accumulation in regions of MR-visible hemorrhagic injury. This has not previously been reported. Prominent increased PET signal in one subject with a history of multiple prior concussions suggests the possibility that these results correspond to acute and/or chronic tauopathy. This would be relevant to understanding the pathogenesis of CTE, which to date has proven difficult to image using PET, and which remains impossible to diagnose in vivo. However, alternate explanations for 18F-MK6240 accumulation in regions of traumatic hemorrhage such as tracer extravasation through a damaged blood-brain barrier (BBB) and off-target radiotracer binding must be considered.
Tracer extravasation
18F-MK6240 is highly BBB permeable [a general requirement for brain PET radiotracers (Pike, 2009)] and does not require BBB breakdown to enter brain parenchyma. Arterial spin labeling (ASL) MRI showed decreased blood flow to regions of radiotracer accumulation (data not shown) which would be expected to decrease tracer delivery. Other PET studies of acute TBI show decreased radiotracer accumulation in regions of hemorrhagic injury (Langfitt et al., 1986). We therefore do not think focal radiotracer accumulation can be attributed to tracer leakage or extravasation.
Binding to blood products
While 18F-MK6240 is considered specific for neurofibrillary tau, autoradiography has shown weak binding to blood products (Aguero et al., 2019; Malarte et al., 2021). Consistent with this, all three subjects showed radiotracer accumulation outside the brain, in regions of extra-axial hemorrhage. This cannot represent parenchymal tau deposition, and confirms that off-target binding to blood products must be considered when interpreting 18F-MK6240 PET. Additional studies e.g., autoradiography and immunohistochemical studies in human tissue obtained at surgery or postmortem and from animal models are needed to clarify the tissue target of 18F-MK6240 in the setting of hemorrhage.
Tauopathy
In Subject #1, PET signal extends into brain parenchyma and well beyond regions of MRI-visible hemorrhage, and we question whether tracer binding to blood products can account fully for these findings. We propose that tau PET signal in this subject with a history of multiple prior concussion, scanned shortly after complicated mild TBI, corresponds, at least in part, to on-target binding of 18F-MK6240 to acutely and/or chronically deposited neurofibrillary tau. While tau tangles are not generally considered to form quickly after TBI, this has been demonstrated in teenaged athletes who died days after suffering concussions, with tau localized to regions of microhemorrhage (McKee et al., 2014; Tagge et al., 2018). This finding in concussion, along with the perivascular location of chronically-deposited tau in CTE (McKee et al., 2014) support the theory that traumatic microvascular injury and release of blood components into brain parenchyma is important to the pathophysiology of tauopathy after TBI (Michalicova et al., 2017; Sandsmark et al., 2019). Much additional neuroimaging and other work is needed to determine if our PET finding of tau radiotracer accumulation surrounding hemorrhagic contusion also supports this theory (vs. corresponding solely to off-target binding) and understand relevant factors, e.g. whether this finding was detectable in one subject because of a predisposing history of multiple prior concussions, injury features, genetic and/or other factors, and whether PET abnormalities resolve over time. The absence of similar tau PET findings in subjects scanned later after injury (Ayubcha et al., 2021; Marklund et al., 2021) suggest this apparent lesion may be transient; follow-up scans are planned for 1 year after injury.
Conclusion
18F-MK6240 tau PET imaging performed shortly after complicated mild TBI in three subjects revealed areas of focal tracer accumulation in regions of traumatic hemorrhage consistent with 1) off-target radiotracer binding to blood products and/or 2) post-traumatic tau deposition. Both explanations are relevant to advancing understanding of tauopathy in TBI and CTE. It is notable that evidence of possible parenchymal tauopathy was present only in one subject with a history of multiple prior concussion. Additional study is needed to clarify the tissue target of 18F-MK6240 PET in the setting of TBI complicated by intracranial bleeding, and to assess its potential utility in understanding how processes occurring acutely after TBI, such as release and deposition of tau and blood from damaged axons and vessels, may relate to future neurodegeneration.
Data availability statement
The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.
Ethics statement
The studies involving human participants were reviewed and approved by Weill Cornell Medicine IRB. The patients/participants provided their written informed consent to participate in this study.
Author contributions
TB wrote the manuscript. TB and MdL contributed to study conceptualization and design. TB, SNN, XHW, YL, LZ, GCC, HR, JPD, EKF, and QRR contributed to image analysis. CS and SM contributed to data acquisition. All authors contributed to data interpretation, reviewing and revising, and approved the final version of this manuscript.
Funding
This research was supported by NIH grants R56NS111052 and RF1AG057570.
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
References
Aguero, C., Dhaynaut, M., Normandin, M. D., Amaral, A. C., Guehl, N. J., Neelamegam, R., et al. (2019). Autoradiography validation of novel tau PET tracer [F-18]-MK-6240 on human postmortem brain tissue. Acta. Neuropathol. Commun. 7, 1–15. doi: 10.1186/s40478-019-0686-6
Ayubcha, C., Moghbel, M., Borja, A. J., Newberg, A., Werner, T. J., Alavi, A., et al. (2021). Tau imaging in head injury. PET Clin. 16, 249–260. doi: 10.1016/j.cpet.2020.12.009
Langfitt, T. W., Obrist, W. D., Alavi, A., Grossman, R. I., Zimmerman, R., Jaggi, J., et al. (1986). Computerized tomography, magnetic resonance imaging, and positron emission tomography in the study of brain trauma: preliminary observations. J. Neurosurg. 64, 760–767. doi: 10.3171/jns.1986.64.5.0760
Malarte, M.-., L, Nordberg, A., and Lemoine, L. (2021). Characterization of MK6240, a tau PET tracer, in autopsy brain tissue from Alzheimer's disease cases. Eur. J. Nucl. Med. Mol. Imaging. 48, 1093–1102. doi: 10.1007/s00259-020-05035-y
Marklund, N., Vedung, F., Lubberink, M., Tegner, Y., Johansson, J., Blennow, K., et al. (2021). Tau aggregation and increased neuroinflammation in athletes after sports-related concussions and in traumatic brain injury patients–A PET/MR study. Neuroimage Clin. 30, 102665. doi: 10.1016/j.nicl.2021.102665
Marshall, L. F., Marshall, S. B., Klauber, M. R., van Berkum Clark, M., Eisenberg, H. M., Jane, J. A., et al. (1991). A new classification of head injury based on computerized tomography. Spec. Suppl. 75, S14–S20. doi: 10.3171/sup.1991.75.1s.0s14
McKee, A. C., Daneshvar, D. H., Alvarez, V. E., and Stein, T. D. (2014). The neuropathology of sport. Acta. Neuropathol. 127, 29–51. doi: 10.1007/s00401-013-1230-6
Michalicova, A., Banks, W. A., Legath, J., and Kovac, A. (2017). Tauopathies-focus on changes at the neurovascular unit. Curr. Alzheimer Res. 14, 790–801. doi: 10.2174/1567205014666170203143336
Pike, V. W. (2009). PET radiotracers: crossing the blood–brain barrier and surviving metabolism. Trends. Pharmacol. Sci. 30, 431–440. doi: 10.1016/j.tips.2009.05.005
Sandsmark, D. K., Bashir, A., Wellington, C. L., and Diaz-Arrastia, R. (2019). Cerebral microvascular injury: a potentially treatable endophenotype of traumatic brain injury-induced neurodegeneration. Neuron. 103, 367–379. doi: 10.1016/j.neuron.2019.06.002
Stern, R. A., Adler, C. H., Chen, K., Navitsky, M., Luo, J., Dodick, D. W., et al. (2019). Tau positron-emission tomography in former national football league players. N. Engl. J. Med. 380, 1716–1725. doi: 10.1056/NEJMoa1900757
Tagge, C. A., Fisher, A. M., Minaeva, O. V., Gaudreau-Balderrama, A., Moncaster, J. A., Zhang, X. L., et al. (2018). Concussion, microvascular injury, and early tauopathy in young athletes after impact head injury and an impact concussion mouse model. Brain 141, 422–458. doi: 10.1093/brain/awx350
Tahmi, M., Bou-Zeid, W., and Razlighi, Q. R. (2019). A fully automatic technique for precise localization and quantification of Amyloid-β PET scans. J. Nucl. Med. 60, 1771–1779. doi: 10.2967/jnumed.119.228510
Keywords: traumatic brain injury (TBI), acute TBI, complicated mild TBI, Positron Emission Tomography (PET), Chronic Traumatic Encephalopathy (CTE), neurodegeneration, tau, neurofibrillary tau
Citation: Butler T, Chiang GC, Niogi SN, Wang XH, Skudin C, Tanzi E, Wickramasuriya N, Spiegel J, Maloney T, Pahlajani S, Zhou L, Morim S, Rusinek H, Normandin M, Dyke JP, Fung EK, Li Y, Glodzik L, Razlighi QR, Shah SA and Leon Md (2022) Tau PET following acute TBI: Off-target binding to blood products, tauopathy, or both? Front. Neuroimaging 1:958558. doi: 10.3389/fnimg.2022.958558
Received: 31 May 2022; Accepted: 15 August 2022;
Published: 14 October 2022.
Edited by:
Nicolas Nicastro, Hôpitaux Universitaires de Genève (HUG), SwitzerlandReviewed by:
Victor Montal, Sant Pau Institute for Biomedical Research, SpainSinisa Franjic, Independent Researcher, Osijek, Croatia
Copyright © 2022 Butler, Chiang, Niogi, Wang, Skudin, Tanzi, Wickramasuriya, Spiegel, Maloney, Pahlajani, Zhou, Morim, Rusinek, Normandin, Dyke, Fung, Li, Glodzik, Razlighi, Shah and Leon. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Tracy Butler, dGFiMjAwNiYjeDAwMDQwO21lZC5jb3JuZWxsLmVkdQ==