AUTHOR=Han Ning , Rao Shreyas S., Johnson Jed , Parikh Kunal S., Bradley Patrick A., Lannutti John J., Winter Jessica O. TITLE=Hydrogel–Electrospun Fiber Mat Composite Coatings for Neural Prostheses JOURNAL=Frontiers in Neuroengineering VOLUME=4 YEAR=2011 URL=https://www.frontiersin.org/journals/neuroengineering/articles/10.3389/fneng.2011.00002 DOI=10.3389/fneng.2011.00002 ISSN=1662-6443 ABSTRACT=

Achieving stable, long-term performance of implanted neural prosthetic devices has been challenging because of implantation related neuron loss and a foreign body response that results in encapsulating glial scar formation. To improve neuron–prosthesis integration and form chronic, stable interfaces, we investigated the potential of neurotrophin-eluting hydrogel–electrospun fiber mat (EFM) composite coatings. In particular, poly(ethylene glycol)-poly(ε-caprolactone) (PEGPCL) hydrogel–poly(ε-caprolactone) EFM composites were applied as coatings for multielectrode arrays. Coatings were stable and persisted on electrode surfaces for over 1 month under an agarose gel tissue phantom and over 9 months in a PBS immersion bath. To demonstrate drug release, a neurotrophin, nerve growth factor (NGF), was loaded in the PEGPCL hydrogel layer, and coating cytotoxicity and sustained NGF release were evaluated using a PC12 cell culture model. Quantitative MTT assays showed that these coatings had no significant toxicity toward PC12 cells, and neurite extension at day 7 and 14 confirmed sustained release of NGF at biologically significant concentrations for at least 2 weeks. Our results demonstrate that hydrogel–EFM composite materials can be applied to neural prostheses to improve neuron–electrode proximity and enhance long-term device performance and function.