AUTHOR=Stanic Karen , Quiroz Alonso , Lemus Carmen G. , Wichmann Ignacio A. , Corvalán Alejandro H. , Owen Gareth I. , Opazo Juan C. , Concha Miguel L. , Amigo Julio D. 

TITLE=Expression of RPRM/rprm in the Olfactory System of Embryonic Zebrafish (Danio rerio)

JOURNAL=Frontiers in Neuroanatomy

VOLUME=12

YEAR=2018

URL=https://www.frontiersin.org/journals/neuroanatomy/articles/10.3389/fnana.2018.00023

DOI=10.3389/fnana.2018.00023

ISSN=1662-5129

ABSTRACT=<p>The Reprimo (<italic>RPRM</italic>) family is composed of highly conserved single-exon genes. The expression pattern of this gene family has been recently described during zebrafish (<italic>Danio rerio</italic>) embryogenesis, and primarily locates in the nervous system. Its most characterized member, <italic>RPRM</italic>, which duplicated to give rise <italic>rprma</italic> and <italic>rprmb</italic> in the fish lineage, is known to act as a tumor-suppressor gene in mammalian models. Here, we describe in detail the spatiotemporal expression of three <italic>rprm</italic> genes (<italic>rprma, rprmb</italic>, and <italic>rprm</italic>l) within distinct anatomical structures in the developing peripheral and central nervous system. In the zebrafish, <italic>rprma</italic> mRNA is expressed in the olfactory placodes (OP) and olfactory epithelium (OE), <italic>rprmb</italic> is observed in the tectum opticum (TeO) and trigeminal ganglion (Tg), whereas <italic>rprml</italic> is found primarily in the telencephalon (Tel). At protein level, RPRM is present in a subset of cells in the OP, and neurons in the OE, TeO, hindbrain and sensory peripheral structures. Most importantly, the expression of <italic>RPRM</italic> has been conserved between teleosts and mammals. Thus, we provide a reference dataset describing the expression patterns of <italic>RPRM</italic> gene products during zebrafish and mouse development as a first step to approach the physiological role of the <italic>RPRM</italic> gene family.</p>