AUTHOR=Nishimura Kaneyasu , Osaki Hironobu , Tezuka Kotaro , Nakashima Daisuke , Numata Shintaro , Masamizu Yoshito TITLE=Recent advances and applications of human brain models JOURNAL=Frontiers in Neural Circuits VOLUME=18 YEAR=2024 URL=https://www.frontiersin.org/journals/neural-circuits/articles/10.3389/fncir.2024.1453958 DOI=10.3389/fncir.2024.1453958 ISSN=1662-5110 ABSTRACT=

Recent advances in human pluripotent stem cell (hPSC) technologies have prompted the emergence of new research fields and applications for human neurons and brain organoids. Brain organoids have gained attention as an in vitro model system that recapitulates the higher structure, cellular diversity and function of the brain to explore brain development, disease modeling, drug screening, and regenerative medicine. This progress has been accelerated by abundant interactions of brain organoid technology with various research fields. A cross-disciplinary approach with human brain organoid technology offers a higher-ordered advance for more accurately understanding the human brain. In this review, we summarize the status of neural induction in two- and three-dimensional culture systems from hPSCs and the modeling of neurodegenerative diseases using brain organoids. We also highlight the latest bioengineered technologies for the assembly of spatially higher-ordered neural tissues and prospects of brain organoid technology toward the understanding of the potential and abilities of the human brain.