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BRIEF RESEARCH REPORT article

Front. Neural Circuits
Volume 18 - 2024 | doi: 10.3389/fncir.2024.1441280

Hyaluronidase induced matrix remodeling contributes to long-term synaptic changes

Provisionally accepted
Rostislav Sokolov Rostislav Sokolov 1*Viktoriya Krut' Viktoriya Krut' 1Vsevolod Belousov Vsevolod Belousov 2Andrei Rozov Andrei Rozov 2Irina Mukhina Irina Mukhina 3
  • 1 Pirogov Russian National Research Medical University, Moscow, Russia
  • 2 Federal State Budgetary Institution “Federal Center for Brain and Neurotechnologies” of the Federal Medical and Biological Agency, Moscow, Moscow Oblast, Russia
  • 3 Privolzhsky Research Medical University (PIMU), Nizhny Novgorod, Nizhny Novgorod Oblast, Russia

The final, formatted version of the article will be published soon.

    Extracellular brain space contains water, dissolved ions, and multiple other signaling molecules.The neural extracellular matrix (ECM) is also a significant component of the extracellular space.ECM is synthetized by neurons, astrocytes, and other types of cells. Hyaluronan, hyaluronic acid polymer, is a key component of ECM. The functions of hyaluronan include barrier functions and signaling. Here we investigate physiological processes during the acute phase of enzymatic ECM removal. We found that hyaluronidase, an ECM removal agent, triggers simultaneous membrane depolarization and sharp calcium influx into neurons. Spontaneous action potential firing frequency increased rapidly after ECM destruction in interneurons, but not pyramidal neurons. Hyaluronidase dependent calcium entry can be blocked by a selective antagonist of N-methyl-D-aspartate (NMDA) receptors, revealing these receptors as the main player in the observed phenomenon. Additionally, here we demonstrate increased NMDA-dependent longterm potentiation at CA3-to-CA1 synapses during the acute phase of ECM removal. These findings suggest hyaluronan is a significant synaptic player.

    Keywords: hippocampal neurons, extracellular brain matrix, synaptic plasticity, NMDAreceptors, Hyaluronidase

    Received: 30 May 2024; Accepted: 16 Dec 2024.

    Copyright: © 2024 Sokolov, Krut', Belousov, Rozov and Mukhina. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Rostislav Sokolov, Pirogov Russian National Research Medical University, Moscow, Russia

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.